{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T08:11:59Z","timestamp":1792656719177,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T00:00:00Z","timestamp":1500508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Aspergillus fumigatus<\/jats:italic> is a saprophytic fungus that can cause lethal invasive aspergillosis in immunocompromised patients. Recent studies have shown that Eurasian and North American populations of <jats:italic>A.\u00a0fumigatus<\/jats:italic> often consist of genetically diverse strains. However, very little is known about African populations of <jats:italic>A.\u00a0fumigatus<\/jats:italic>. Here, we characterise the genetic diversity and triazole susceptibility of <jats:italic>A.\u00a0fumigatus<\/jats:italic> in Cameroon, West Africa. A total of 495 soil samples were obtained from nine collection sites in three Cameroonian regions. Nine microsatellite markers were used to genotype all 51 identified <jats:italic>A.\u00a0fumigatus<\/jats:italic> isolates. In vitro susceptibility to itraconazole and voriconazole was tested using micro broth dilution. The 51 Cameroonian <jats:italic>A.\u00a0fumigatus<\/jats:italic> isolates belonged to 45 genotypes. Consistent with recombination, 32 of 36 possible pairwise loci combinations are phylogenetically incompatible. Interestingly, evidence for geographic sub\u2010structuring was found within Cameroon and the sub\u2010population with the most evidence of recombination was also the least susceptible sub\u2010population to the triazole antifungals tested. Furthermore, the Cameroonian sample was significantly differentiated from those in Eurasia and North America. Overall, our results indicate the genetic uniqueness of Cameroonian <jats:italic>A.\u00a0fumigatus<\/jats:italic> populations and that additional novel genetic diversity likely exist in other parts of Africa.<\/jats:p>","DOI":"10.1111\/myc.12655","type":"journal-article","created":{"date-parts":[[2017,7,21]],"date-time":"2017-07-21T01:56:45Z","timestamp":1500602205000},"page":"739-748","source":"Crossref","is-referenced-by-count":24,"title":["Evidence of unique genetic diversity in <i>Aspergillus fumigatus<\/i> isolates from Cameroon"],"prefix":"10.1111","volume":"60","author":[{"given":"Eta E.","family":"Ashu","sequence":"first","affiliation":[{"name":"Department of Biology McMaster University  Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Korfanty","sequence":"additional","affiliation":[{"name":"Department of Biology McMaster University  Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2915-2780","authenticated-orcid":false,"given":"Jianping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biology McMaster University  Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00274-07"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00055-08"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07528"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693780903095414"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.512301"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.09.019"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2011.05364.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12494"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00019-17"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019688"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2016.05.008"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030075"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.5.2264-2266.1999"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2015.0460"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.326.5957.1173"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku259"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1796-4"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4112-4120.2005"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.10.4384"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2005.02553.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2004.02396.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-11-94"},{"key":"e_1_2_7_24_1","unstructured":"Clinical and Laboratory Standards Institute.Reference method for broth dilution antifungal susceptibility testing of filamentous fungi. Standard\u2010second edition. Approved Standard\u2010Second Edition. Wayne PA USA: CLSI M38\u2010A2; 2002."},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762009000300005"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.130193297"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052871"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00536-15"},{"key":"e_1_2_7_29_1","first-page":"A4458","article-title":"Azole resistance in Aspergillus fumigatus in the Netherlands\u2013increase due to environmental fungicides","volume":"156","author":"Verweij PE","year":"2012","journal-title":"Ned Tijdschr Geneeskd"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ885"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00100-15"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/142.4.1119"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2007.03.003"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003743"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(99)00061-6"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12655","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12655","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T20:57:32Z","timestamp":1696021052000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,20]]},"references-count":34,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1111\/myc.12655"],"URL":"https:\/\/doi.org\/10.1111\/myc.12655","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,20]]}}}