{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T12:38:45Z","timestamp":1754483925253,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T00:00:00Z","timestamp":1505779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), Spain"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Previous studies have evaluated the action of gentamicin against <jats:italic>Malassezia pachydermatis<\/jats:italic>. The aim of this study was to evaluate in vitro susceptibility of <jats:italic>M.\u00a0pachydermatis<\/jats:italic> to the aminoglycosides\u2014 gentamicin, tobramycin, netilmicin and framycetin. The minimum inhibitory concentration (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>) of gentamicin was determined following methods M27\u2010A3 microdilution and Etest<jats:sup>\u00ae<\/jats:sup>. The Etest<jats:sup>\u00ae<\/jats:sup> was used to determine the minimum inhibitory concentration (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>) of the tobramycin and netilmicin. The Kirby\u2010Bauer test was used to determine the antibiotic susceptibility to the framycetin. The <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>50 and <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>90 were 8.12\u00a0\u03bcg\/mL and 32.5\u00a0\u03bcg\/mL by microdilution method for gentamicin. The <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>50, determined by the Etest<jats:sup>\u00ae<\/jats:sup>, was 8\u00a0\u03bcg\/mL for gentamicin and netilmicin and 64\u00a0\u03bcg\/mL for tobramycin. The <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>90 was 16 and 32\u00a0\u03bcg\/mL for gentamicin and netilmicin respectively. The <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>90 was outside of the detectable limits for tobramycin. To framycetin, 28 strains (40%) of the 70\u00a0<jats:italic>M.\u00a0pachydermatis<\/jats:italic> isolates tested showed a diameter of 22\u00a0mm, 22 strains (31.42%) showed a diameter of 20\u00a0mm, 16 strains showed a diameter of \u2264 18\u00a0mm, and only 5.71% of the isolates showed a diameter of \u2265 22\u00a0mm. This study provides evidence of high in vitro activity of the aminoglycosides\u2014gentamicin, tobramycin, netilmicin and framycetin against <jats:italic>M.\u00a0pachydermatis<\/jats:italic>. For gentamicin Etest<jats:sup>\u00ae<\/jats:sup> showed similar values of <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>50 y <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>90 that the obtained by microdilution method. We considered Etest<jats:sup>\u00ae<\/jats:sup> method could be a good method for these calculations with aminoglycosides.<\/jats:p>","DOI":"10.1111\/myc.12665","type":"journal-article","created":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T05:46:43Z","timestamp":1505800003000},"page":"796-799","source":"Crossref","is-referenced-by-count":2,"title":["Susceptibility of <i>Malassezia pachydermatis<\/i> to aminoglycosides"],"prefix":"10.1111","volume":"60","author":[{"given":"Freddy Alejandro","family":"Silva","sequence":"first","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA) Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnolia","family":"Conde\u2010Felipe","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda Animal Facultad de Veterinaria Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inmaculada","family":"Rosario","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA) Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Otilia","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda Animal Facultad de Veterinaria Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Real","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA) Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraya","family":"D\u00e9niz","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA) Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e9lix","family":"Acosta","sequence":"additional","affiliation":[{"name":"Parque Cient\u00edfico Tecnol\u00f3gico Marino Universidad de Las Palmas de Gran Canaria \u2010 Ctra.  Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Padilla","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA) Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0397-4244","authenticated-orcid":false,"given":"Bego\u00f1a","family":"Acosta\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA) Universidad de Las Palmas de Gran Canaria  Arucas Las Palmas Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-280X.1999.00237.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700403"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822009000200002"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2009.12.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2001.tb00312.x"},{"key":"e_1_2_7_7_1","first-page":"41","article-title":"Malassezia Estado del conocimiento y perspectivas en su estudio","volume":"38","author":"Giusiano E","year":"2006","journal-title":"Rev Arg Micol"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.12.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2005.05.006"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70218-8"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00091-14"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822003000400015"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.09.008"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.04.034"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-015-9913-2"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2009.11.003"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12427"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00399623"},{"volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts: Approved Standard \u2010 Third Edition. CLSI Document M27\u2010A3","year":"2008","author":"Clinical and Laboratory Standards Institute","key":"e_1_2_7_20_1"},{"volume-title":"Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts; Approved Guideline","year":"2009","author":"Clinical Laboratory Standards Institute","key":"e_1_2_7_21_1"},{"key":"e_1_2_7_22_1","first-page":"77","article-title":"Susceptibility to antifungal agents of Malassezia pachydermatis isolates from dogs","volume":"4","author":"Coutinho S","year":"2001","journal-title":"Pol J Vet Sci"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.3.535-540.1995"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.7.1.107"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.13-0433"},{"key":"e_1_2_7_26_1","first-page":"72","article-title":"Genotyping and antifungal susceptibility testing of multiple Malassezia pachydermatis isolates from otitis and dermatitis cases in pets: is it really worth the effort?","volume":"54","author":"\u00c1lvarez\u2010P\u00e9rez S","year":"2016","journal-title":"Med Mycol"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2011.01025.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2007.00996.x"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12665","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12665","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T10:01:59Z","timestamp":1696759319000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12665"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,19]]},"references-count":27,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/myc.12665"],"URL":"https:\/\/doi.org\/10.1111\/myc.12665","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"type":"print","value":"0933-7407"},{"type":"electronic","value":"1439-0507"}],"subject":[],"published":{"date-parts":[[2017,9,19]]}}}