{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:02:51Z","timestamp":1789624971866,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The diagnosis of invasive pulmonary aspergillosis (<jats:styled-content style=\"fixed-case\">IPA<\/jats:styled-content>) increasingly relies on non\u2010culture\u2010based biomarkers in bronchoalveolar lavage (<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>) fluid. The <jats:italic>Aspergillus<\/jats:italic> lateral flow device (<jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content>) is a rapid immunoassay that uses a novel <jats:italic>Aspergillus<\/jats:italic> monoclonal antibody to gain specificity. The objective of the study is to compare specificity and sensitivity of the prototype <jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> and the galactomannan (<jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content>) enzyme immunoassay in <jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content> fluid in high\u2010risk patients. A total of 114 BAL samples from 106 patients at high risk for IPA were studied: 8 patients had proven\/probable <jats:styled-content style=\"fixed-case\">IPA<\/jats:styled-content>, 16 had possible <jats:styled-content style=\"fixed-case\">IPA<\/jats:styled-content> and 82 did not have <jats:styled-content style=\"fixed-case\">IPA<\/jats:styled-content>. In patients with proven\/probable <jats:styled-content style=\"fixed-case\">IPA<\/jats:styled-content>, specificity of <jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> was 94% and <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content> was 89%; sensitivity of <jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> was 38% and <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content> was 75%. Negative predictive value (<jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content>) for <jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> was 94% and for <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content> was 98%; positive predictive value (<jats:styled-content style=\"fixed-case\">PPV<\/jats:styled-content>) was 38% for both tests. The use of anti\u2010mould prophylaxis did not affect specificity but resulted in decreased <jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content> of both <jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content>. Union and intersection analysis showed no improvement in the performance by using both tests. Among patients at risk for <jats:styled-content style=\"fixed-case\">IPA<\/jats:styled-content>, the diagnostic performance of <jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content> fluid appears comparable; specificity is high, but sensitivity of both <jats:styled-content style=\"fixed-case\">LFD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content> is poor.<\/jats:p>","DOI":"10.1111\/myc.12694","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T07:02:11Z","timestamp":1505372531000},"page":"4-10","source":"Crossref","is-referenced-by-count":18,"title":["Testing the performance of a prototype lateral flow device using bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in high\u2010risk patients"],"prefix":"10.1111","volume":"61","author":[{"given":"Caroline G.","family":"Castillo","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases Department of Internal Medicine University of Michigan Health System  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol A.","family":"Kauffman","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Internal Medicine University of Michigan Health System  Ann Arbor MI USA"},{"name":"Veterans Affairs Ann Arbor Healthcare System University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Biostatistics University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Biostatistics University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie M.","family":"Agozino","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory University of Michigan Health System  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3175-0512","authenticated-orcid":false,"given":"Marisa H.","family":"Miceli","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Internal Medicine University of Michigan Health System  Ann Arbor MI 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