{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:10:23Z","timestamp":1759774223164,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Fungal rhinosinusitis (FRS) has a worldwide distribution, comprises distinct clinical entities but is mostly due to <jats:italic>Aspergillus<\/jats:italic> among which <jats:italic>Aspergillus\u00a0fumigatus<\/jats:italic> plays a major role in European countries. Although, there is accumulating evidence for the emergence of environmentally acquired\u2010azole resistance in <jats:italic>A.\u00a0fumigatus<\/jats:italic> (such as <jats:styled-content style=\"fixed-case\">TR<\/jats:styled-content><jats:sub>34<\/jats:sub>\/L98H) in various clinical settings, there is few data for patients with FRS. In this study, we aimed to investigate the prevalence of <jats:italic>A.\u00a0fumigatus<\/jats:italic> azole resistance due to <jats:styled-content style=\"fixed-case\">TR<\/jats:styled-content><jats:sub>34<\/jats:sub>\/L98H in a multicentre cohort of patients with FRS. One hundred and thirty\u2010seven patients with FRS admitted between 2002 and 2016 at four French medical centres were retrospectively enrolled. Clinical and mycological findings were collected. <jats:italic>Aspergillus fumigatus<\/jats:italic> and the <jats:styled-content style=\"fixed-case\">TR<\/jats:styled-content><jats:sub>34<\/jats:sub>\/L98H alteration conferring azole resistance were investigated directly from clinical samples using the commercial <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IVD<\/jats:styled-content> marked Myco<jats:styled-content style=\"fixed-case\">GENIE<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup> <jats:italic>A.\u00a0fumigatus<\/jats:italic> real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay. Fungal ball was the more frequent clinical form (n\u00a0=\u00a0118). Despite the presence of fungal hyphae at direct microscopic examination, mycological cultures remained negative for 83 out of the 137 patients (60.6%). The <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay proved to be useful allowing the identification of <jats:italic>A.\u00a0fumigatus<\/jats:italic> and etiological diagnosis in 106 patients (77.4%) compared with 44 patients (32.1%) when using culture as the reference method. Importantly, neither <jats:styled-content style=\"fixed-case\">TR<\/jats:styled-content><jats:sub>34<\/jats:sub> nor L98H alterations were evidenced.<\/jats:p>","DOI":"10.1111\/myc.12702","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T16:06:38Z","timestamp":1505750798000},"page":"30-34","source":"Crossref","is-referenced-by-count":17,"title":["<scp>PCR<\/scp>\u2010based detection of <i>Aspergillus fumigatus<\/i> and absence of azole resistance due to <scp>TR<\/scp><sub>34<\/sub>\/L98H in a french multicenter cohort of 137 patients with fungal rhinosinusitis"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5859-6105","authenticated-orcid":false,"given":"Florent","family":"Morio","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie\u2010Mycologie Institut de Biologie CHU Nantes  Nantes France"},{"name":"D\u00e9partement de Parasitologie et Mycologie M\u00e9dicale EA1155 IICiMed Institut de Recherche en Sant\u00e9 2 Universit\u00e9 de Nantes, Nantes Atlantique Universit\u00e9s  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Dannaoui","sequence":"additional","affiliation":[{"name":"Dynamyc Research Group UPEC, EnvA, UPE  Cr\u00e9teil, Paris France"},{"name":"Facult\u00e9 de M\u00e9decine APHP H\u00f4pital Europ\u00e9en Georges Pompidou Unit\u00e9 de Parasitologie\u2010Mycologie Service de Microbiologie Universit\u00e9 Paris\u2010Descartes  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taieb","family":"Chouaki","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie\u2010Mycologie CHU Amiens  Amiens France"},{"name":"EA 4666 CAP\u2010Sant\u00e9 (FED 4231)  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estelle","family":"Cateau","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie\u2010Mycologie CHU Poitiers  Poitiers France"},{"name":"Ecologie et Biologie des Interactions UMR CNRS 7267 Equipe Microbiologie de l'Eau Universit\u00e9 de Poitiers  Poitiers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Malard","sequence":"additional","affiliation":[{"name":"Service d'ORL et de chirurgie cervicofaciale CHU Nantes  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Bonfils","sequence":"additional","affiliation":[{"name":"Service d'ORL et de chirurgie cervicofaciale H\u00f4pital Europ\u00e9en Georges Pompidou  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyril","family":"Page","sequence":"additional","affiliation":[{"name":"Service d'ORL et de chirurgie cervicofaciale CHU Amiens  Amiens France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier","family":"Dufour","sequence":"additional","affiliation":[{"name":"Service d'ORL et de chirurgie cervicofaciale CHU Poitiers  Poitiers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Cottrel","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Parasitologie et Mycologie M\u00e9dicale EA1155 IICiMed Institut de Recherche en Sant\u00e9 2 Universit\u00e9 de Nantes, Nantes Atlantique Universit\u00e9s  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamic","family":"Erwan","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Parasitologie et Mycologie M\u00e9dicale EA1155 IICiMed Institut de Recherche en Sant\u00e9 2 Universit\u00e9 de Nantes, Nantes Atlantique Universit\u00e9s  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose\u2010Anne","family":"Lavergne","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie\u2010Mycologie Institut de Biologie CHU Nantes  Nantes France"},{"name":"D\u00e9partement de Parasitologie et Mycologie M\u00e9dicale EA1155 IICiMed Institut de Recherche en Sant\u00e9 2 Universit\u00e9 de Nantes, Nantes Atlantique Universit\u00e9s  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Le Pape","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie\u2010Mycologie Institut de Biologie CHU Nantes  Nantes France"},{"name":"D\u00e9partement de Parasitologie et Mycologie M\u00e9dicale EA1155 IICiMed Institut de Recherche en Sant\u00e9 2 Universit\u00e9 de Nantes, Nantes Atlantique Universit\u00e9s  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20520"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599815627786"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500235728"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/684835"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myw041"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw326"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ885"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.6.1364"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05088-11"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031801"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70265-8"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2201.150978"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir179"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00213-11"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/23744235.2016.1193791"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00428"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01032-17","article-title":"Molecular diagnosis of invasive aspergillosis and detection of azole resistance by a newly commercialized PCR kit","author":"Dannaoui E","year":"2017","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00520-17"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00411-17"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw323"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03216-14"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01594-15"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks160"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2106.140717"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw259"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05077-11"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1093\/mmy\/myv029","article-title":"Prospective evaluation of azole resistance in Aspergillus fumigatus clinical isolates in France","volume":"53","author":"Choukri F","year":"2015","journal-title":"Med Mycol"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12702","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12702","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T01:26:52Z","timestamp":1695950812000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,26]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1111\/myc.12702"],"URL":"https:\/\/doi.org\/10.1111\/myc.12702","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"type":"print","value":"0933-7407"},{"type":"electronic","value":"1439-0507"}],"subject":[],"published":{"date-parts":[[2017,9,26]]}}}