{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:06:56Z","timestamp":1778641616827,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,22]],"date-time":"2017-11-22T00:00:00Z","timestamp":1511308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A clear differentiation between pneumonia due to<jats:italic>Pneumocystis jirovecii<\/jats:italic>and \u201ccolonisation\u201d is required for optimal case management. A quantification of fungal burden using major surface glycoprotein (<jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content>) gene\u2010based real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>was undertaken for the same. Lower respiratory tract samples collected from 104 patients of clinically suspected Pneumocystis pneumonia (<jats:styled-content style=\"fixed-case\">PCP<\/jats:styled-content>) were subjected to quantitative<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>using<jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content>gene. Based on whether or not the cases were treated for<jats:styled-content style=\"fixed-case\">PCP<\/jats:styled-content>, the efficacy of<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>to differentiate between \u201cdiseased\u201d and \u201ccolonised\u201d was evaluated. Standard curve of plasmid\u2010cloned gene and receiver operating characteristic curve defined a cut\u2010off of Ct\u00a0\u2264\u00a025 to diagnose<jats:styled-content style=\"fixed-case\">PCP<\/jats:styled-content>and Ct within 26\u201039.3 range to depict colonisation. The sensitivity, specificity, positive predictive value (<jats:styled-content style=\"fixed-case\">PPV<\/jats:styled-content>) and negative predictive value (<jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content>) of the<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>was 100%, each for diagnosing<jats:styled-content style=\"fixed-case\">PCP<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content>\u2010gene\u2010based<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>is a robust tool for the reliable differentiation of Pneumocystis pneumonia from colonisation.<\/jats:p>","DOI":"10.1111\/myc.12708","type":"journal-article","created":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T14:01:21Z","timestamp":1506348081000},"page":"96-103","source":"Crossref","is-referenced-by-count":9,"title":["Reliable differentiation of Pneumocystis pneumonia from Pneumocystis colonisation by quantification of Major Surface Glycoprotein gene using real\u2010time polymerase chain reaction"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-9253","authenticated-orcid":false,"given":"Shivaprakash M.","family":"Rudramurthy","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6740-0493","authenticated-orcid":false,"given":"Megha","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhubala","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pankaj","family":"Rawat","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anup","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshmishree","family":"Venkatesan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritesh","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meenu","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1555-3807","authenticated-orcid":false,"given":"Arunaloke","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2005.00072.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.42"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.08-2859"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.118.3.704"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03400.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-002-0758-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200301030-00010"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-012-0441-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3333-3335.2004"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45528-0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1099\/00222615-45-2-146","article-title":"Imbalance between Pneumocystis carinii cysts and trophozoites in bronchoalveolar lavage fluid from patients with pneumocystosis receiving prophylaxis","volume":"45","author":"Tamburrini E","year":"1996","journal-title":"J Med Microbiol"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2989-2993.2002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-1960-3"},{"key":"e_1_2_7_15_1","first-page":"704","article-title":"Characteristics and clinical relevance of the quantitative touch\u2010down major surface glycoprotein polymerase chain reaction in the diagnosis of Pneumocystis pneumonia","volume":"49","author":"Chumpitazi BFF","year":"2011","journal-title":"Med Mycol"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03174-15"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02072-15"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46552-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.627-639.2001"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1517\/17530059.2012.722080"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06036-11"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.06.009"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.3760\/cma.j.issn.0366-6999.20122506","article-title":"Use of real\u2010time polymerase chain reaction for the diagnosis of Pneumocystis pneumonia in immunocompromised patients: a meta\u2010analysis","volume":"126","author":"Summah H","year":"2013","journal-title":"Chin Med J (Engl)"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/mmy\/myw051","article-title":"A misleading false\u2010negative result of Pneumocystis real\u2010time PCR assay due to a rare punctual mutation\u202f: a French multicenter study","volume":"55","author":"Le Gal S","year":"2017","journal-title":"Med Mycol"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01480-14"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.081270"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keq314"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir996"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01554-13"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06066-11"},{"key":"e_1_2_7_31_1","first-page":"321","article-title":"Use of different primer directed sequence amplification by polymerase chain reaction for identification of Pneumocystis jirovecii in clinical samples","volume":"50","author":"Gupta R","year":"2008","journal-title":"Indian J Chest Dis Allied Sci"},{"key":"e_1_2_7_32_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2016.01413","article-title":"Copy number variation of mitochondrial DNA genes in pneumocystis jirovecii according to the fungal load in bal specimens","volume":"7","author":"Valero C","year":"2016","journal-title":"Front Microbiol"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2015.0213"},{"key":"e_1_2_7_34_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1155\/2015\/138787","article-title":"Development and validation of a Pneumocystis jirovecii real\u2010time polymerase chain reaction assay for diagnosis of Pneumocystis pneumonia","volume":"26","author":"Church DL","year":"2015","journal-title":"Can J Infect Dis Med Microbiol"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12708","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12708","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T06:53:04Z","timestamp":1719471184000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,22]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/myc.12708"],"URL":"https:\/\/doi.org\/10.1111\/myc.12708","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,22]]}}}