{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:50:12Z","timestamp":1791557412258,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T00:00:00Z","timestamp":1510704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Deep trichophytosis is relatively uncommon. The infection of the bearded area is also known as sycosis barbae or tinea barbae and can be caused by various fungal species, most often zoophilic fungi. We report on an 80\u2010year\u2010old male patient with severe sycosis barbae who had no animal contact and was treated with systemic antibiosis without improvement. Microbial and mycological investigations using swabs from oozing lesions revealed<jats:italic>Staphylococcus haemolyticus<\/jats:italic>and<jats:italic>Candida parapsilosis<\/jats:italic>. Histology demonstrated fungal elements in hair follicles. Paraffin\u2010embedded material was subjected to further mycological analysis. For molecular diagnostics DNA was prepared from paraffin sections for real\u2010time polymerase chain reaction (RT\u2010PCR). For sequencing, DNA was isolated from paraffin\u2010embedded skin tissue and the ITS region of the rDNA was selected. Sequencing of the ITS2 region of rRNA revealed a 100% accordance with<jats:italic>Trichophyton<\/jats:italic>(<jats:italic>T<\/jats:italic>.)<jats:italic>verrucosum<\/jats:italic>. Treatment with oral terbinafine achieved a complete remission. Sycosis barbae is an important differential diagnosis for infections of the bearded area. Nucleic acid amplification techniques (NAAT) are more and more used for direct examination of dermatophytes in clinical samples, eg<jats:italic>T<\/jats:italic>.\u00a0<jats:italic>verrucosum<\/jats:italic>. NAAT are also used as culture confirmation tests for identification of rare dermatophytes like<jats:italic>T<\/jats:italic>.\u00a0<jats:italic>verrucosum<\/jats:italic>. Today, singleplex and multiplex quantitative real\u2010time PCR (qRT\u2010PCR) assays for the detection of the most common dermatophytes including<jats:italic>T<\/jats:italic>.\u00a0<jats:italic>verrucosum<\/jats:italic>in clinical specimens are available. Recently, an ITS2 PCR assay has been successfully used for direct detection of<jats:italic>T<\/jats:italic>.\u00a0<jats:italic>verrucosum<\/jats:italic>in paraffin\u2010embedded formalin\u2010fixed skin tissue. The PCR is fast and highly specific. The sensitivity of direct molecular detection of the dermatophytes both in native clinical material, and in paraffin\u2010embedded skin tissue can been increased.<\/jats:p>","DOI":"10.1111\/myc.12719","type":"journal-article","created":{"date-parts":[[2017,10,30]],"date-time":"2017-10-30T06:42:39Z","timestamp":1509345759000},"page":"152-158","source":"Crossref","is-referenced-by-count":13,"title":["Deep facial mycosis due to<i>Trichophyton verrucosum<\/i>\u2014molecular genetic identification of the dermatophyte in paraffin\u2010embedded tissue\u2014case report and review of the literature"],"prefix":"10.1111","volume":"61","author":[{"given":"Uwe","family":"Wollina","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergology Municipal Hospital Dresden Academic Teaching Hospital Friedrichstadt Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gesine","family":"Hansel","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Municipal Hospital Dresden Academic Teaching Hospital Friedrichstadt Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Uhrla\u00df","sequence":"additional","affiliation":[{"name":"Laboratory for Medial Microbiology M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constanze","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Laboratory for Medial Microbiology M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Sch\u00f6nlebe","sequence":"additional","affiliation":[{"name":"Institute of Pathology \u201cGeorg Schmorl\u201d Municipal Hospital Dresden Academic Teaching Hospital Friedrichstadt Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uta\u2010Christina","family":"Hipler","sequence":"additional","affiliation":[{"name":"Department of Dermatology Medical Faculty University of Jena Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4990-3345","authenticated-orcid":false,"given":"Pietro","family":"Nenoff","sequence":"additional","affiliation":[{"name":"Laboratory for Medial Microbiology M\u00f6lbis Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,15]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"161","article-title":"Sycosis of the beard (tinea barbae). 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