{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T10:33:48Z","timestamp":1778754828697,"version":"3.51.4"},"reference-count":10,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T00:00:00Z","timestamp":1514246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7164255"],"award-info":[{"award-number":["7164255"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Onychomycosis is a common fungal infection in dermatology clinics. The commonly used diagnostic method, potassium hydroxide (<jats:styled-content style=\"fixed-case\">KOH<\/jats:styled-content>) direct microscopy, does not have a high\u2010positive detection rate. Therefore, a new diagnostic method is needed to confirm onychomycosis. The objective of this research was to use fluorescent brightener 85 (<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content>85), a new fluorescent reagent for fungi detection, and to evaluate this new method based on the diagnostic positive rate, accuracy and efficiency in suspected onychomycosis compared with the<jats:styled-content style=\"fixed-case\">KOH<\/jats:styled-content>method. Subungual debris was collected from clinically suspected onychomycosis cases and divided into two parts. The parts were examined by<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content>85 and 10%<jats:styled-content style=\"fixed-case\">KOH<\/jats:styled-content>solution, respectively. In total, 108 patients with suspected onychomycosis were recruited. The positive rates of<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content>85 and<jats:styled-content style=\"fixed-case\">KOH<\/jats:styled-content>were 88.9% and 55.6%, respectively. The patients showed significant statistical differences (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a0.001). Compared with the<jats:styled-content style=\"fixed-case\">KOH<\/jats:styled-content>method, the<jats:styled-content style=\"fixed-case\">FB<\/jats:styled-content>85 fluorescent method was an accurate and efficient method with a higher positive rate. This method could increase the diagnostic accuracy and efficiency of onychomycosis detection.<\/jats:p>","DOI":"10.1111\/myc.12733","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T17:38:14Z","timestamp":1511977094000},"page":"279-282","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of a new fluorescent reagent, fluorescent brightener 85, for the diagnosis of suspected onychomycosis compared with potassium hydroxide"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1222-2535","authenticated-orcid":false,"given":"Xueping","family":"Yue","sequence":"first","affiliation":[{"name":"Department of Dermatology Beijing Tian Tan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology &amp; Venereology Peking University First Hospital and Research Center for Medical Mycology Peking University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Tian Tan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Beijing Tian Tan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuling","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Beijing Tian Tan Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.7547\/14-063.1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ced.12593"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2015.03.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.06.022"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.4103\/0377-4929.200049","article-title":"Efficacy of in\u2010house\u00a0fluorescent\u00a0stain for fungus","volume":"60","author":"Kirani KR","year":"2017","journal-title":"Indian J Pathol Microbiol"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2012.07.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1067\/S0190-9622(03)01480-4"},{"key":"e_1_2_9_9_1","first-page":"12","article-title":"Observe the spitzenk(o)rper of Trichosporon asahii","volume":"6","author":"Lv YT","year":"2013","journal-title":"Journal of Practical Dermatology"},{"key":"e_1_2_9_10_1","first-page":"68","article-title":"An observation of the rapid lifts of Trichosporon asahii","volume":"6","author":"Lv YT","year":"2013","journal-title":"Journal of Practical Dermatology"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/15572536.2006.11832788"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12733","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12733","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T06:17:37Z","timestamp":1719641857000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12733"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,26]]},"references-count":10,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/myc.12733"],"URL":"https:\/\/doi.org\/10.1111\/myc.12733","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,26]]}}}