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The Campinas University Hospital (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">UNICAMP<\/jats:styled-content>) receives high\u2010risk patients susceptible to opportunistic infections but there have been no reports of resistant <jats:italic>A.\u00a0fumigatus<\/jats:italic>. This study aimed to assess the susceptibility profile of <jats:italic>Aspergillus<\/jats:italic> isolates, specifically looking for azole resistance. <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> and \u03b2\u2010tubulin <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequencing was performed on 228 sequential clinical isolates. Broth microdilution susceptibility testing was performed for all isolates. <jats:italic>A.\u00a0fumigatus<\/jats:italic> represented 74% of the isolates followed by <jats:italic>Aspergillus flavus<\/jats:italic> (12%). Nine <jats:italic>A.\u00a0fumigatus<\/jats:italic> isolates from 9 different patients showed high <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> values to at least 1 azole, but <jats:italic>cyp51A<\/jats:italic> polymorphisms were detected in only 6 isolates and none correlated with known resistance mutations. The most troubling observation was that the minimum inhibitory concentration for amphotericin B was elevated (\u22652\u00a0mg\u00a0L<jats:sup>\u22121<\/jats:sup>) in 87% of patients with <jats:italic>A.\u00a0flavus<\/jats:italic> isolates and 43% with <jats:italic>Aspergillus\u00a0fumigatus<\/jats:italic> isolates. Given that amphotericin B is used to treat azole\u2010resistant infections, these data highlight the need for continuous surveillance in <jats:italic>Aspergillus<\/jats:italic> for all antifungal resistance to implement correct treatment strategies for the management of these pathogens.<\/jats:p>","DOI":"10.1111\/myc.12759","type":"journal-article","created":{"date-parts":[[2018,2,22]],"date-time":"2018-02-22T07:36:42Z","timestamp":1519285002000},"page":"360-365","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Surveillance for azoles resistance in <i>Aspergillus<\/i> spp. highlights a high number of amphotericin B\u2010resistant isolates"],"prefix":"10.1111","volume":"61","author":[{"given":"Franqueline","family":"Reichert\u2010Lima","sequence":"first","affiliation":[{"name":"Clinical Pathology Department School of Medical Sciences State University of Campinas  Sao Paulo 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn R.","family":"Lockhart","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5095-5054","authenticated-orcid":false,"given":"Ang\u00e9lica Zaninelli","family":"Schreiber","sequence":"additional","affiliation":[{"name":"Clinical Pathology Department School of Medical Sciences State University of Campinas  Sao Paulo 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