{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:49:20Z","timestamp":1787856560231,"version":"build-2784847793"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T00:00:00Z","timestamp":1528675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Research Grant from Palacky University Olomouc, Czech Republic","award":["IGA_LF_2017_031"],"award-info":[{"award-number":["IGA_LF_2017_031"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,8]]},"abstract":"Summary<\/jats:title>Detection of serum galactomannan (GM<\/jats:styled-content>) and (1,3)\u2010\u03b2\u2010d<\/jats:sc>\u2010glucan (BG<\/jats:styled-content>) is considered useful for non\u2010culture diagnosis of invasive pulmonary aspergillosis (IPA<\/jats:styled-content>) in neutropenic patients. Only few studies evaluated these seromarkers in non\u2010neutropenic patients suspected of having IPA<\/jats:styled-content>. The aim of this study was to evaluate both tests together with the Aspergillus fumigatus<\/jats:italic>\u2010specific serum IgG and IgA (IgAG<\/jats:styled-content>) test for serological IPA<\/jats:styled-content> diagnosis in non\u2010neutropenic patients. Sera from 87 patients suspected of having IPA<\/jats:styled-content> were retrospectively analysed. Patients were categorised into groups of proven IPA<\/jats:styled-content> (n\u00a0=\u00a010), putative IPA<\/jats:styled-content> (n\u00a0=\u00a031) and non\u2010IPA<\/jats:styled-content> colonisation (n\u00a0=\u00a046). When the GM<\/jats:styled-content>, BG<\/jats:styled-content> and IgAG<\/jats:styled-content> assays were used for patients included in the study, the sensitivity\/specificity\/positive predictive value (PPV<\/jats:styled-content>)\/negative predictive value (NPV<\/jats:styled-content>) were 48.8%\/91.3%\/83.3%\/66.7%, 82.9%\/73.9%\/73.9%\/82.9% and 75.6%\/95.7%\/93.9%\/81.5%, respectively. Thus, the highest specificity and PPV<\/jats:styled-content> were confirmed for the IgAG<\/jats:styled-content> assay. Improvements in the sensitivity and NPV<\/jats:styled-content> were achieved by \u201cat least one positive\u201d analysis with the GM<\/jats:styled-content> and BG<\/jats:styled-content> assays, with the sensitivity\/specificity\/PPV<\/jats:styled-content>\/NPV<\/jats:styled-content> values being 85.0%\/69.6%\/71.4%\/84.2%. Nevertheless, the highest sensitivity and NPV<\/jats:styled-content> were achieved by the \u201cat least one positive\u201d analysis combining the GM<\/jats:styled-content>, BG<\/jats:styled-content> and IgAG<\/jats:styled-content> tests (97.6% and 96.8%, respectively). The involvement of the IgAG<\/jats:styled-content> assay could improve IPA<\/jats:styled-content> diagnosis in non\u2010neutropenic patients by increasing the sensitivity and NPV<\/jats:styled-content> when combined with the GM<\/jats:styled-content> or BG<\/jats:styled-content> assays. Furthermore, improvement was achieved by combining the GM<\/jats:styled-content>, BG<\/jats:styled-content> and IgAG<\/jats:styled-content> assays using the \u201cat least one positive test\u201d strategy, especially if doubt exists.<\/jats:p>","DOI":"10.1111\/myc.12765","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T17:57:03Z","timestamp":1522000623000},"page":"576-586","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Diagnostic value of serum galactomannan, (1,3)\u2010\u03b2\u2010d<\/scp>\u2010glucan, and Aspergillus fumigatus<\/i>\u2010specific IgA and IgG assays for invasive pulmonary aspergillosis in non\u2010neutropenic patients"],"prefix":"10.1111","volume":"61","author":[{"given":"R.","family":"Dobias","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Mycology, Bacteriology and Mycology Institute of Public Health in Ostrava Ostrava Czech Republic"},{"name":"Department of Microbiology Faculty of Medicine and Dentistry Palacky University Olomouc Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Jaworska","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Mycology, Bacteriology and Mycology Institute of Public Health in Ostrava Ostrava Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Tomaskova","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine and Dentistry Palacky University Olomouc Olomouc Czech Republic"},{"name":"Center of Health Services Institute of Public Health in Ostrava Ostrava Czech Republic"},{"name":"Department of Epidemiology and Public Health Faculty of Medicine University of Ostrava Ostrava Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kanova","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Public Health Faculty of Medicine University of Ostrava Ostrava Czech Republic"},{"name":"Department of Anesthesiology and Intensive Care Medicine University Hospital Ostrava Ostrava Czech Republic"},{"name":"Department of Intensive Medicine, Emergency Medicine and Forensic Studies University of Ostrava Ostrava Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Lyskova","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine and Dentistry Palacky University Olomouc Olomouc Czech Republic"},{"name":"Laboratory of Mycology Department of Parasitology, Mycology and Mycobacteriology Prague Public Health Institute in Usti nad Labem Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Vrba","sequence":"additional","affiliation":[{"name":"Lung Department Krnov Combined Medical Facility Krnov Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Holub","sequence":"additional","affiliation":[{"name":"Lung Department Krnov Combined Medical Facility Krnov Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Svobodov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine and Dentistry Palacky University Olomouc Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5361-8125","authenticated-orcid":false,"given":"P.","family":"Hamal","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine and Dentistry Palacky University Olomouc Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Raska","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Medicine and Dentistry Palacky University Olomouc Olomouc Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16971"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-017-0143-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01143.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03015.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00001011"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000043"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx032"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500064771"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2012.02283.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.53.2381"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-014-3254-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1086\/503427"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq206"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780801978968"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200704-606OC"},{"key":"e_1_2_7_17_1","first-page":"e4345","article-title":"Bronchoalveolar lavage (BAL) for research; obtaining adequate sample yield","volume":"85","author":"Collins AM","year":"2014","journal-title":"J Vis Exp"},{"key":"e_1_2_7_18_1","first-page":"112","article-title":"Grouping of Aspergillus species with exoantigens","volume":"4","author":"Sekhon AS","year":"1986","journal-title":"Diagn Immunol"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.10035"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2004.01035.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.98.11061274"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1086\/588660"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1179\/acb.2004.037"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201111-1978OC"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.12.5957-5962.2005"},{"key":"e_1_2_7_26_1","first-page":"148","article-title":"Assessment of Platelia Aspergillus enzyme immunoassay for the diagnosis of invasive aspergillosis","volume":"40","author":"Lai CC","year":"2007","journal-title":"J Microbiol Immunol Infect"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2012.657672"},{"key":"e_1_2_7_28_1","first-page":"929","article-title":"The diagnostic performance of galactomannan detection for invasive pulmonary aspergillosis in non\u2010neutropenic hosts","volume":"39","author":"Lin PC","year":"2016","journal-title":"Chin J Tuberculosis Respir Dis"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.521523"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis493"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802308454"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2011.638319"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03567-13"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-016-0011-x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrc.2016.06.026"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18040862"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00506-08"},{"key":"e_1_2_7_38_1","first-page":"48","article-title":"Detection of 1,3\u2010\u03b2\u2010D glucan for diagnosis of invasive fungal infections in hematooncological patients: usefulness for screening of invasive mycosis and for confirmation of galactomannan positive results","volume":"15","author":"Racil Z","year":"2009","journal-title":"Klin Mikrobiol Infekc Lek"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-007-0791-z"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-015-1140-1"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc3488"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200401-093OC"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12765","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12765","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T12:19:11Z","timestamp":1695039551000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12765"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,11]]},"references-count":41,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1111\/myc.12765"],"URL":"https:\/\/doi.org\/10.1111\/myc.12765","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,11]]},"assertion":[{"value":"2017-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}