{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:25:43Z","timestamp":1791285943191,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,6,20]],"date-time":"2018-06-20T00:00:00Z","timestamp":1529452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Aspergillosis in falcons may be associated with high mortality and difficulties in clinical and laboratory diagnosis. We previously cloned an immunogenic protein, Afmp1p, in <jats:italic>Aspergillus fumigatus<\/jats:italic> and showed that anti\u2010Afmp1p antibodies were present in human patients with <jats:italic>A. fumigatus<\/jats:italic> infections. In this study, we hypothesise that a similar Afmp1p\u2010based enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) could be applied to serodiagnose falcon aspergillosis. A specific polyclonal antibody was first generated to detect falcon serum IgY. Horseradish peroxidase\u2010conjugate of this antibody was then used to measure anti\u2010Afmp1p antibodies in sera collected from falcons experimentally infected with <jats:italic>A. fumigatus<\/jats:italic>, and the performance of the Afmp1p\u2010based <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> was evaluated using sera from healthy falcons and falcons with documented <jats:italic>A. fumigatus<\/jats:italic> infections. All four experimentally infected falcons developed culture\u2010 and histology\u2010proven invasive aspergillosis. Anti\u2010Afmp1p antibodies were detected in their sera. For the Afmp1p\u2010based <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, the mean\u00a0\u00b1\u00a0SD <jats:styled-content style=\"fixed-case\">OD<\/jats:styled-content><jats:sub>450\u00a0nm<\/jats:sub> using sera from 129 healthy falcons was 0.186\u00a0\u00b1\u00a00.073. Receiver operating characteristics curve analysis showed an absorbance cut\u2010off value of 0.407. One negative serum gave an absorbance outside the normal range, giving a specificity of 99.2%. For the 12 sera from falcons with confirmed aspergillosis, nine gave absorbance values \u2265 cut\u2010off, giving a sensitivity of 75%. The Afmp1p\u2010based <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> is useful for serodiagnosis of falcons with aspergillosis.<\/jats:p>","DOI":"10.1111\/myc.12776","type":"journal-article","created":{"date-parts":[[2018,4,3]],"date-time":"2018-04-03T07:55:26Z","timestamp":1522742126000},"page":"600-609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Serodiagnosis of aspergillosis in falcons (<i>Falco<\/i> spp.) by an Afmp1p\u2010based enzyme\u2010linked immunosorbent assay"],"prefix":"10.1111","volume":"61","author":[{"given":"Ulrich","family":"Wernery","sequence":"first","affiliation":[{"name":"Central Veterinary Research Laboratory  Dubai United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6705-2866","authenticated-orcid":false,"given":"Chi\u2010Ching","family":"Tsang","sequence":"additional","affiliation":[{"name":"Department of Microbiology Li Ka Shing Faculty of Medicine The University of Hong Kong  Pokfulam Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiana","family":"Hebel","sequence":"additional","affiliation":[{"name":"F3 Falcons R  Dubai United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Damerau","sequence":"additional","affiliation":[{"name":"University of Applied Sciences  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Kinne","sequence":"additional","affiliation":[{"name":"Central Veterinary Research Laboratory  Dubai United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Piao","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Microbiology Li Ka Shing Faculty of Medicine The University of Hong Kong  Pokfulam Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"K\u00fcspert","sequence":"additional","affiliation":[{"name":"Falcon Center GbR  Helvesiek Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2290-3182","authenticated-orcid":false,"given":"Ka\u2010Fai","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Microbiology Li Ka Shing Faculty of Medicine The University of Hong Kong  Pokfulam Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Joseph","sequence":"additional","affiliation":[{"name":"Central Veterinary Research Laboratory  Dubai United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaolong","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Microbiology Li Ka Shing Faculty of Medicine The University of Hong Kong  Pokfulam Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha","family":"Raghavan","sequence":"additional","affiliation":[{"name":"Central Veterinary Research Laboratory  Dubai United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3521-5688","authenticated-orcid":false,"given":"James Y. 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