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Still, there is no consensus about recognition of antigens from <jats:italic>P.\u00a0lutzii<\/jats:italic> by antibodies directed to other <jats:italic>Paracoccidioides<\/jats:italic> species, which can have great impact on Paracoccidioidomycosis (<jats:styled-content style=\"fixed-case\">PCM<\/jats:styled-content>) diagnosis. Current research investigated soluble protein\/carbohydrate epitopes from <jats:italic>P.\u00a0lutzii <\/jats:italic><jats:styled-content style=\"fixed-case\">LDR<\/jats:styled-content>2, <jats:italic>Paracoccidioides\u00a0restrepiensis<\/jats:italic> B339 and <jats:italic>Paracoccidioides\u00a0americana <\/jats:italic><jats:styled-content style=\"fixed-case\">LDR<\/jats:styled-content>3 recognised by IgG directed to <jats:italic>Paracoccidioides\u00a0brasiliensis<\/jats:italic>. Cell free antigens (<jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>) were analysed by: (a)silver and periodic acid\u2010Schiff staining of <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>; (b)immunoblot (<jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content>) with rabbit IgG anti\u2010<jats:italic>P.\u00a0brasiliensis<\/jats:italic> Pb18; (c)<jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> with a pool of <jats:styled-content style=\"fixed-case\">PCM<\/jats:styled-content> patients\u2019 sera before and after treatment with sodium metaperiodate (<jats:styled-content style=\"fixed-case\">SMP<\/jats:styled-content>) to oxidise carbohydrate epitopes. Both rabbit and human immune sera recognised several antigens of <jats:italic>P.\u00a0lutzii <\/jats:italic><jats:styled-content style=\"fixed-case\">LDR<\/jats:styled-content>2, <jats:italic>P.\u00a0restrepiensis<\/jats:italic> B339 and <jats:italic>P.\u00a0americana <\/jats:italic><jats:styled-content style=\"fixed-case\">LDR<\/jats:styled-content>3. <jats:italic>P.\u00a0lutzii<\/jats:italic>'s gp43 was not observed in <jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content> or silver\/<jats:styled-content style=\"fixed-case\">PAS<\/jats:styled-content> staining. <jats:styled-content style=\"fixed-case\">SMP<\/jats:styled-content> treatment affected reactions with all 3 <jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>s, but more intensely with antigens from <jats:italic>P.\u00a0lutzii <\/jats:italic><jats:styled-content style=\"fixed-case\">LDR<\/jats:styled-content>2. In conclusion, antibodies directed to <jats:italic>P.\u00a0brasiliensis<\/jats:italic> recognised antigens from <jats:italic>P.\u00a0lutzii <\/jats:italic><jats:styled-content style=\"fixed-case\">LDR<\/jats:styled-content>2. The use of any of the recognised antigens in a broad spectrum diagnostic model for <jats:italic>Paracoccidioides<\/jats:italic> species complex needs to be further investigated.<\/jats:p>","DOI":"10.1111\/myc.12819","type":"journal-article","created":{"date-parts":[[2018,6,25]],"date-time":"2018-06-25T11:26:20Z","timestamp":1529925980000},"page":"826-832","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Polyclonal antibodies to <i>Paracoccidioides brasiliensis<\/i> are able to recognise antigens from different strains from <i>Paracoccidioides<\/i> species complex, including <i>Paracoccidioides lutzii <\/i><scp>LDR<\/scp>2"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1597-6853","authenticated-orcid":false,"given":"Adriane","family":"Lenhard\u2010Vidal","sequence":"first","affiliation":[{"name":"State University of Londrina  Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4190-8835","authenticated-orcid":false,"given":"Jo\u00e3o P.","family":"Assolini","sequence":"additional","affiliation":[{"name":"State University of Londrina  Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franciele A. 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