{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T16:39:25Z","timestamp":1780072765803,"version":"3.54.0"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T00:00:00Z","timestamp":1535414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004160","name":"Mazandaran University of Medical Sciences","doi-asserted-by":"publisher","award":["1560"],"award-info":[{"award-number":["1560"]}],"id":[{"id":"10.13039\/501100004160","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Accurate diagnosis of mucormycosis, a life\u2010threatening fungal infection, remains a challenge for physicians.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To identify the causative <jats:italic>Mucorales<\/jats:italic> in fresh clinical samples and formalin\u2010fixed paraffin\u2010embedded (<jats:styled-content style=\"fixed-case\">FFPE<\/jats:styled-content>) samples of patients with proven mucormycosis by molecular method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>Fresh clinical samples of patients with proven mucormycosis according to the <jats:styled-content style=\"fixed-case\">EORTC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MSG<\/jats:styled-content> criteria admitted between 2015 and 2017 and histopathologically proven <jats:styled-content style=\"fixed-case\">FFPE<\/jats:styled-content> archives collected during 2004\u20102007 and 2015\u20102017 from Mazandaran University\u2010affiliated hospitals of northern Iran were included. Seminested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> targeting the 18S <jats:styled-content style=\"fixed-case\">rDNA<\/jats:styled-content> of <jats:italic>Mucorales<\/jats:italic> and <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> region was performed, and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> products were then sequenced.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>While culture was positive only in 5 of 9 (56%) of fresh specimen cases, <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was positive in all 9 (100%) histologically proven mucormycosis. Ten of 18 (56%) <jats:styled-content style=\"fixed-case\">FFPE<\/jats:styled-content> samples were <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010positive. Overall, <jats:italic>Mucorales <\/jats:italic><jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was positive in 19 of 27 (70%) samples. <jats:italic>Mucorales<\/jats:italic> species were <jats:italic>Rhizopus arrhizus<\/jats:italic> in 16 (84%) cases, <jats:italic>R.\u00a0arrhizus<\/jats:italic>\/<jats:italic>Amylomyces rouxii<\/jats:italic> in 2 (10.5%) cases and <jats:italic>Rhizopus stolonifer<\/jats:italic> in one case (5.5%). Among 27 mucormycosis cases, 25 (93%) cases were rhinocerebral, and 2 (7%) cases were disseminated. Diabetes mellitus (74%) and neutropaenia (63%) were the main risk factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Seminested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> targeting 18S <jats:styled-content style=\"fixed-case\">rDNA<\/jats:styled-content> region of <jats:italic>Mucorales<\/jats:italic> is useful for identification of the causative agents of mucormycosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12837","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T07:53:16Z","timestamp":1533801196000},"page":"909-915","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Identification of <i>Mucorales<\/i> in patients with proven invasive mucormycosis by polymerase chain reaction in tissue samples"],"prefix":"10.1111","volume":"61","author":[{"given":"Nahid","family":"Gholinejad\u2010Ghadi","sequence":"first","affiliation":[{"name":"Student Research Committee School of Medicine Mazandaran University of Medical Sciences  Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3094-8436","authenticated-orcid":false,"given":"Tahereh","family":"Shokohi","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology School of Medicine Mazandaran University of Medical Sciences  Sari Iran"},{"name":"Invasive Fungi Research Centre (IFRC) Mazandaran University of Medical Sciences  Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Seifi","sequence":"additional","affiliation":[{"name":"Student Research Committee School of Medicine Mazandaran University of Medical Sciences  Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed 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