{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T14:09:28Z","timestamp":1775052568819,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T00:00:00Z","timestamp":1539475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Galderma R&D"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Onychomycosis is the most prevalent nail disease and is mainly caused by two dermatophyte species <jats:italic>Trichophyton rubrum<\/jats:italic> and <jats:italic>Trichophyton interdigitale<\/jats:italic> with a frequency in the range of 80% and 20%, respectively. The secreted protease Sub6 of the subtilisin family, which was never detected in vitro growth conditions, was found to be a robust marker of onychomycosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this work was to detect tinea unguium using anti\u2010Sub6 monoclonal antibodies in proteins extracted from clinical nail samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We produced monoclonal antibodies in mice using recombinant Sub6 as an antigen. Selected monoclonal antibodies were tested by Western blot analysis and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> on protein extracts from onychomycosis samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several monoclonal antibodies used to quantify Sub6 in proteins extracted from clinical nail samples were produced and characterised. We showed that these antibodies were very specific and allowed the detection of <jats:italic>T.\u00a0rubrum<\/jats:italic> and <jats:italic>T.\u00a0interdigitale<\/jats:italic> in onychomycosis. Sub6 was detected in clinical samples infected by <jats:italic>T.\u00a0rubrum<\/jats:italic> and not detected in nails with trauma and other diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anti\u2010Sub6 monoclonal antibodies could be useful for a rapid diagnosis of tinea unguium and\/or therapeutic survey of dermatophyte in onychomycosis by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> or an immunochromatography device such as a strip test.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12843","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T04:27:55Z","timestamp":1535603275000},"page":"32-40","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detection of <i>Trichophyton rubrum<\/i> and <i>Trichophyton interdigitale<\/i>\u00a0in onychomycosis using monoclonal antibodies against Sub6 (Tri r 2)"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3521-6413","authenticated-orcid":false,"given":"Bruno","family":"M\u00e9hul","sequence":"first","affiliation":[{"name":"Galderma R&amp;D  Sophia\u2010Antipolis France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niccolo","family":"de Coi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Centre Hospitalier Universitaire Vaudois  Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Grundt","sequence":"additional","affiliation":[{"name":"Galderma R&amp;D  Sophia\u2010Antipolis France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"Genette","sequence":"additional","affiliation":[{"name":"Galderma R&amp;D  Sophia\u2010Antipolis France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes J.","family":"Voegel","sequence":"additional","affiliation":[{"name":"Galderma R&amp;D  Sophia\u2010Antipolis France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0695-4003","authenticated-orcid":false,"given":"Michel","family":"Monod","sequence":"additional","affiliation":[{"name":"Department of Dermatology Centre Hospitalier Universitaire Vaudois  Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,14]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"703","article-title":"Revision of the dermatophyte species and the nomenclature of these fungi","volume":"13","author":"Monod M","year":"2017","journal-title":"Rev Med 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