{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T03:20:42Z","timestamp":1784949642085,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T00:00:00Z","timestamp":1539648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In recent years, human keratitis caused by fungal plant pathogens has become more common. Biofilm is a structure that confers adaptations and virulence to fungi in keratitis. <jats:italic>Neoscytalidium<\/jats:italic> spp. are phytopathogenic and recently have been recognised as a human pathogen, using biofilm formation as a virulence factor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was isolation, identification (at the species level) and characterisation of a new fungal keratitis agent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/methods<\/jats:title><jats:p>The fungus was isolated from a 67\u2010year\u2010old male patient with a corneal ulcer. Biofilm formation and structure were evaluated by colorimetric methods and microscopy. To identify the fungus, morphological characteristics were examined and a phylogenetic analysis was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and conclusions<\/jats:title><jats:p>We report the identification of a fungus, a member of the genus <jats:italic>Neoscytalidium<\/jats:italic> which is associated with human keratitis. Phylogenetic analysis and morphological observations on conidiogenous cells, which occur only in arthric chains in aerial mycelium and the coelomycetous synasexual morph is absent, identified a new species, <jats:italic>Neoscytalidium oculus<\/jats:italic> sp. nov. The fungus formed biofilm at a concentration of 1\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0conidia\/mL, during 96\u00a0hours of incubation at 37\u00b0C, and also manifested haemolysis and melanin production. This is the first report in Latin America of a new species of <jats:italic>Neoscytalidium<\/jats:italic> from a clinical isolate has been identified.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12849","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T08:58:01Z","timestamp":1536224281000},"page":"62-72","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Identification and biofilm development by a new fungal keratitis aetiologic agent"],"prefix":"10.1111","volume":"62","author":[{"given":"Rosa Paulina","family":"Calvillo\u2010Medina","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Prote\u00f3mica Instituto de Oftalmolog\u00eda \u201cFundaci\u00f3n Conde de Valenciana\u201d  Mexico City M\u00e9xico"},{"name":"Laboratorio de Microbiolog\u00eda Molecular Universidad Aut\u00f3noma de Quer\u00e9taro  Santiago de Quer\u00e9taro M\u00e9xico"},{"name":"Consorcio de Estructura de Prote\u00ednas Instituto Nacional de Medicina Gen\u00f3mica  Mexico City M\u00e9xico"},{"name":"Departamento de Microbiolog\u00eda Escuela Nacional de Ciencias Biol\u00f3gicas Instituto Polit\u00e9cnico Nacional  Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Mart\u00ednez\u2010Neria","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmolog\u00eda Hospital General \u201cDr. Manuel Gea Gonz\u00e1lez\u201d  Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Mena\u2010Portales","sequence":"additional","affiliation":[{"name":"Instituto de Ecolog\u00eda y Sistem\u00e1tica  La Habana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Barba\u2010Escoto","sequence":"additional","affiliation":[{"name":"Sustainable Intensification Program M\u00e9xico Edo. de M\u00e9xico International Maize and Wheat Improvement Center (CIMMYT)  Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Raymundo","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda Escuela Nacional de Ciencias Biol\u00f3gicas Instituto Polit\u00e9cnico Nacional  Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Campos\u2010Guill\u00e9n","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular Universidad Aut\u00f3noma de Quer\u00e9taro  Santiago de Quer\u00e9taro M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George H.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Biology Emory University  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Pablo","family":"Reyes\u2010Grajeda","sequence":"additional","affiliation":[{"name":"Consorcio de Estructura de Prote\u00ednas Instituto Nacional de Medicina Gen\u00f3mica  Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Alberto","family":"Gonz\u00e1lez\u2010y\u2010Merchand","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda Escuela Nacional de Ciencias Biol\u00f3gicas Instituto Polit\u00e9cnico Nacional  Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1836-8738","authenticated-orcid":false,"given":"Victor Manuel","family":"Bautista\u2010de Lucio","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Prote\u00f3mica Instituto de Oftalmolog\u00eda \u201cFundaci\u00f3n Conde de Valenciana\u201d  Mexico City M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-013-0139-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.eye.6700557"},{"key":"e_1_2_7_4_1","first-page":"174","article-title":"Clinical presentation and microbial analyses of contact lens keratitis; 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