{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:30:06Z","timestamp":1790515806320,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T00:00:00Z","timestamp":1539475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The incidence of fungal keratitis has increased in recent years. While the epidemiology and clinical roles of various <jats:italic>Candida<\/jats:italic> and <jats:italic>Fusarium<\/jats:italic> species have been relatively well\u2010identified in infections of the eye, data regarding keratitis caused by <jats:italic>Aspergillus<\/jats:italic> species are scant. Accurate and rapid diagnosis is important for successful management of this infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To present the first molecular epidemiological data from Mexico during a 4\u2010year period of cases admitted with <jats:italic>Aspergillus<\/jats:italic> keratitis to a tertiary care eye institution in Mexico City.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>A total of 25 cases of keratitis were included in the study. <jats:italic>Aspergillus<\/jats:italic> isolates were identified by sequencing the calmodulin gene. Antifungal susceptibility was tested according to CLSI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The aetiological agents belonged to <jats:italic>Aspergillus flavus<\/jats:italic> (n\u00a0=\u00a013), <jats:italic>Aspergillus effusus<\/jats:italic> (n\u00a0=\u00a01), <jats:italic>Aspergillus tamarii<\/jats:italic> (n\u00a0=\u00a04), <jats:italic>Aspergillus sydowii<\/jats:italic> (n\u00a0=\u00a01), <jats:italic>Aspergillus protuberus<\/jats:italic> (n\u00a0=\u00a03) and <jats:italic>Aspergillus terreus<\/jats:italic> (n\u00a0=\u00a03). All strains had low minimum inhibitory concentrations (MICs) of itraconazole and voriconazole (VCZ). Amphotericin B and natamycin showed moderate elevated MICs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Early diagnosis and application of topical VCZ 1% were associated with good outcome. Monitoring of local epidemiological data plays an important role in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12855","type":"journal-article","created":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T13:50:00Z","timestamp":1537969800000},"page":"144-151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Epidemiology of <i>Aspergillus<\/i> species causing keratitis in Mexico"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5206-2647","authenticated-orcid":false,"given":"Abdullah M. S.","family":"Al\u2010Hatmi","sequence":"first","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht The Netherlands"},{"name":"Directorate General of Health Services Ministry of Health  Ibri Oman"},{"name":"Centre of Expertise in Mycology Radboud University Medical Centre \/ Canisius Wilhelmina Hospital  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marino Alcantara","family":"Castro","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Asociacion para Evitar la Ceguera en Mexico Hospital \u201cDr. Luis Sanchez\u2010Bulnes\u201d  Coyoac\u00e1n Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5344-257X","authenticated-orcid":false,"given":"G. Sybren","family":"de Hoog","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute  Utrecht The Netherlands"},{"name":"Centre of Expertise in Mycology Radboud University Medical Centre \/ Canisius Wilhelmina Hospital  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6010-8414","authenticated-orcid":false,"given":"Hamid","family":"Badali","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology Invasive Fungi Research Center (IFRC) School of Medicine Mazandaran University of Medical Sciences  Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Flores","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Asociacion para Evitar la Ceguera en Mexico Hospital \u201cDr. Luis Sanchez\u2010Bulnes\u201d  Coyoac\u00e1n Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Verweij","sequence":"additional","affiliation":[{"name":"Centre of Expertise in Mycology Radboud University Medical Centre \/ Canisius Wilhelmina Hospital  Nijmegen The Netherlands"},{"name":"Department of Medical Microbiology Radboud University Medical Centre  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3253-6080","authenticated-orcid":false,"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[{"name":"Centre of Expertise in Mycology Radboud University Medical Centre \/ Canisius Wilhelmina Hospital  Nijmegen The Netherlands"},{"name":"Department of Medical Microbiology and Infectious Diseases Canisius Wilhelmina Hospital (CWZ)  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia Vanzzini","family":"Zago","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Asociacion para Evitar la Ceguera en Mexico Hospital \u201cDr. Luis Sanchez\u2010Bulnes\u201d  Coyoac\u00e1n Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2016.126"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.86.11.1211"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S10819"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.4.730-797.2003"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-013-0150-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2014-305945"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/643104"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv105"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.185592"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2013.1497"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2008.12.043"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2015.11.004"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2012.99"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01960583"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2014.07.004"},{"key":"e_1_2_6_17_1","volume-title":"Reference Method for Broth Dilution antifungal Susceptibility Testing of filamentous Fungi. Approved Standard M38\u2010A2","author":"Clinical and Laboratory Standards Institute","year":"2008"},{"key":"e_1_2_6_18_1","volume-title":"Atlas of Clinical Fungi","author":"Hoog GS","year":"2011"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2005.07.032"},{"key":"e_1_2_6_20_1","first-page":"57","article-title":"Mycotic keratitis in an eye care hospital in Mexico City","volume":"27","author":"Vanzzini V","year":"2010","journal-title":"Rev Iberoam Micol"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12395"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2013-304013"},{"key":"e_1_2_6_24_1","first-page":"2651","article-title":"Causes of corneal blindness: a multi\u2010center retrospective study","volume":"90","author":"Prabhaswat P","year":"2007","journal-title":"J Med Assoc Thai"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01070-09"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00383-13"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2012.02194.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-016-0089-1"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2018.07.040"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2010.01996.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713680500423671"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2006.09.042"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000151539.92865.3e"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.29386"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.86328"},{"key":"e_1_2_6_36_1","first-page":"1098","article-title":"Efficacy of anti\u2010fungal agents in the cornea. I. A comparative study","volume":"24","author":"O\u2032Day DM","year":"1983","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.125.6.789"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1001\/archophthalmol.2010.102"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv421"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2016.10.009"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2010.09.002"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmyc.12855","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.12855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T13:36:39Z","timestamp":1694698599000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.12855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,14]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/myc.12855"],"URL":"https:\/\/doi.org\/10.1111\/myc.12855","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,14]]},"assertion":[{"value":"2018-08-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}