{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:50:52Z","timestamp":1762005052722,"version":"3.37.3"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T00:00:00Z","timestamp":1542067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81201269"],"award-info":[{"award-number":["81201269"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cryptococcosis is a life\u2010threatening mycosis primarily occurring in adult patients particularly those with immunosuppression such as <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> infection\/<jats:styled-content style=\"fixed-case\">AIDS<\/jats:styled-content>. The number of reported cases of paediatric cryptococcosis has increased in the last decade around the world, including China. However, current information on the characteristics of cryptococcosis in children, particularly the genotypic diversity and antifungal susceptibility of the isolates, is limited. In the present study, a total of 25 paediatric isolates of <jats:italic>Cryptococcus neoformans<\/jats:italic> were genotyped using the <jats:styled-content style=\"fixed-case\">ISHAM<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content> scheme. In vitro susceptibility to antifungal agents of the 22 isolates was tested using the <jats:styled-content style=\"fixed-case\">CLSI<\/jats:styled-content> M27\u2010A3 method. Our analyses revealed that the genotypic diversity of <jats:italic>C.\u00a0neoformans<\/jats:italic> isolates from Chinese paediatric patients was low, with <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content> 5 (80%) and <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content> 31 (12%) being the two major sequence types. Reduced susceptibility to fluconazole (<jats:styled-content style=\"fixed-case\">FLU<\/jats:styled-content>), 5\u2010flucytosine (5\u2010<jats:styled-content style=\"fixed-case\">FC<\/jats:styled-content>) and itraconazole (<jats:styled-content style=\"fixed-case\">ITR<\/jats:styled-content>) was observed among <jats:italic>C.\u00a0neoformans<\/jats:italic> isolates from Chinese paediatric patients, particularly among the <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>5 isolates, which was similar to observations made on <jats:italic>C.\u00a0neoformans<\/jats:italic> isolates from Chinese adult patients. In addition, the majority of isolates (3\/4, 75%) obtained from deceased patients showed decreased antifungal susceptibility, which indicates that further monitoring of antifungal susceptibility of <jats:italic>Cryptococcus<\/jats:italic> isolates is warranted in management of paediatric cryptococcosis.<\/jats:p>","DOI":"10.1111\/myc.12863","type":"journal-article","created":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T06:20:44Z","timestamp":1540016444000},"page":"171-180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Genotypic diversity and antifungal susceptibility of <i>Cryptococcus neoformans<\/i> isolates from paediatric patients in China"],"prefix":"10.1111","volume":"62","author":[{"given":"Nan","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Dermatology Changzheng Hospital Second Military Medical University  Shanghai China"},{"name":"Shanghai Key Laboratory of Medical Mycology Changzheng Hospital Second Military Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0629-9264","authenticated-orcid":false,"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology Changzheng Hospital Second Military Medical University  Shanghai China"},{"name":"Shanghai Key Laboratory of Medical Mycology Changzheng Hospital Second Military Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah M. 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