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Antibody detection, which is faster than the gold standard culture test, hastens the laboratory investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate the role of <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> for antibody detection in the diagnosis of histoplasmosis among <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AIDS<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p>Fifty patients with proven or probable histoplasmosis were included. Clinical, epidemiological and laboratory data were described in the same population after a review of their medical records. <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> was performed using deglycosylated histoplasmin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>About 82% of patients were adult males and the mean age was 39.3\u00a0years. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T lymphocyte count less than 150\u00a0cells\/mm<jats:sup>3<\/jats:sup> was observed in 62% patients. Antibodies against <jats:italic>Histoplasma capsulatum<\/jats:italic> M antigen were detected in 62% of patients, and against both M and H antigens in 28% of individuals. Sera from 10% of patients were nonreactive. Histoplasmosis was the first opportunistic infection in 38% of the cases. Disseminated and pulmonary histoplasmosis occurred in 84% and 16% of patients, respectively. The overall mortality was 16%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> could be useful for the histoplasmosis diagnosis in <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AIDS<\/jats:styled-content> patients because of its easefulness and good sensitivity in a population where antibody production is hampered.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12877","type":"journal-article","created":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T15:38:56Z","timestamp":1545147536000},"page":"261-267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Role of western blot assay for the diagnosis of histoplasmosis in <scp>AIDS<\/scp> patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil"],"prefix":"10.1111","volume":"62","author":[{"given":"Marcos Abreu","family":"Almeida","sequence":"first","affiliation":[{"name":"Instituto Nacional de Infectologia Evandro Chagas Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisandra Serra","family":"Damasceno","sequence":"additional","affiliation":[{"name":"Hospital S\u00e3o Jos\u00e9 de Doen\u00e7as Infecciosas  Fortaleza CE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Vera","family":"Pizzini","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Infectologia Evandro Chagas Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro de Medeiros","family":"Muniz","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Infectologia Evandro Chagas Funda\u00e7\u00e3o Oswaldo Cruz  Rio de Janeiro RJ 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