{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T08:51:06Z","timestamp":1772700666559,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T00:00:00Z","timestamp":1550620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Nieder\u00f6sterreichische Forschungs- und Bildungsges.m.b.H.","award":["NfB LSC13-026"],"award-info":[{"award-number":["NfB LSC13-026"]}]},{"name":"Nieder\u00f6sterreichische Forschungs- und Bildungsges.m.b.H.","award":["LSC16-016"],"award-info":[{"award-number":["LSC16-016"]}]},{"name":"Nieder\u00f6sterreichische Forschungs- und Bildungsges.m.b.H.","award":["K3-G-2\/026-2013"],"award-info":[{"award-number":["K3-G-2\/026-2013"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Candida<\/jats:italic>\u2010associated infections put a significant burden on western healthcare systems. Development of (multi\u2010)resistant fungi can become untreatable and threaten especially vulnerable target groups, such as the immunocompromised.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We assessed antifungal susceptibility and explored possible influence factors of clinical <jats:italic>Candida<\/jats:italic> isolates collected from Austrian hospitals between 2007 and 2016.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thousand three hundred and sixty clinical <jats:italic>Candida<\/jats:italic> spp. isolated from blood cultures were subjected to antifungal susceptibility testing (<jats:styled-content style=\"fixed-case\">AFST<\/jats:styled-content>) in a liquid\u2010handling aided continuous microdilution assay. We tested against fluconazole, voriconazole, posaconazole, itraconazole, isavuconazole, anidulafungin, caspofungin and micafungin according to <jats:styled-content style=\"fixed-case\">EUCAST<\/jats:styled-content> with additional recording of growth curves. We performed rigid quality control on each assay via growth curve assessment and included two standard reference strains. Minimal inhibitory concentrations (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>) were quantified according to <jats:styled-content style=\"fixed-case\">EUCAST<\/jats:styled-content> guideline E.<jats:styled-content style=\"fixed-case\">DEF<\/jats:styled-content> 7.3.1, and susceptibility was evaluated using <jats:styled-content style=\"fixed-case\">EUCAST<\/jats:styled-content> clinical breakpoints.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The isolate collection consisted of <jats:italic>Candida albicans<\/jats:italic> (59%), <jats:italic>C.\u00a0glabrata<\/jats:italic> (19%), <jats:italic>C.\u00a0parapsilosis<\/jats:italic> (9%), <jats:italic>C.\u00a0tropicalis<\/jats:italic> (5%) and <jats:italic>C.\u00a0krusei<\/jats:italic> (3%) and few other <jats:italic>Candida<\/jats:italic> species and fungi (5%). During the observed time period, species abundance and antifungal resistance rates remained constant. Multi\u2010resistance was rare and we found no single isolate which was resistant to both azoles and echinocandins. Within the antifungal resistance profile of our strain collection, we observed clusters along species boundaries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Over the last decade, the distribution of <jats:italic>Candida<\/jats:italic> species and its level of antifungal resistance remained constant in Austria. Our data compare well with other European countries. Principal component analysis of the susceptibility profile of this collection revealed species\u2010specific clusters and substantial intra\u2010species variation, especially for <jats:italic>C.\u00a0glabrata<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12892","type":"journal-article","created":{"date-parts":[[2019,1,12]],"date-time":"2019-01-12T13:09:51Z","timestamp":1547298591000},"page":"357-367","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Antifungal susceptibility of yeast bloodstream isolates collected during a 10\u2010year period in Austria"],"prefix":"10.1111","volume":"62","author":[{"given":"Reinhard","family":"Beyer","sequence":"first","affiliation":[{"name":"Department of Applied Genetics and Cell Biology (DAGZ) University of Natural Resources and Life Sciences  Vienna (BOKU) Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin","family":"Spettel","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine Medical University of Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"Zeller","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine Medical University of Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Lass\u2010Fl\u00f6rl","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology (HMM) Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dagmar","family":"Achleitner","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology University Hospital Salzburg (SALK)  Salzburg 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