{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:16:01Z","timestamp":1790417761295,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T00:00:00Z","timestamp":1554336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Candida auris<\/jats:italic> is an emerging multidrug\u2010resistant yeast associated with invasive infection in healthcare settings. Recently, <jats:italic>C\u00a0auris<\/jats:italic> cases in the United States have been detected in 11 states with the majority of cases in New York, New Jersey and Illinois. Rapid and accurate identification of <jats:italic>C\u00a0auris<\/jats:italic> is critical for patient care and the implementation of public health measures to control the spread of infection. Our aim was to develop and validate a rapid <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction method using the Roche Mag<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content> Pure 96 instrument and a TaqMan real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay for reliable, high\u2010throughput identification of <jats:italic>C\u00a0auris<\/jats:italic>. We evaluated 247 patient dermal swab samples previously analysed by culture\/<jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>. The diagnostic sensitivity and specificity were 93.6% and 97.2%, respectively. The assay was highly reproducible with a detection limit of 1 <jats:italic>C\u00a0auris <\/jats:italic><jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>\/10\u00a0\u03bcL. A receiver operating characteristic curve analysis of the real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> data showed an area of 0.982 under the curve, with a <jats:styled-content style=\"fixed-case\"><jats:italic>C<\/jats:italic><jats:sub>T<\/jats:sub><\/jats:styled-content> cut\u2010off value of \u226437.0. The turnaround time from <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction to real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> results was approximately 200\u00a0samples\/day. In conclusion, we successfully validated a rapid and high\u2010throughput method for accurate and reproducible identification of <jats:italic>C\u00a0auris<\/jats:italic> with a significantly reduced turnaround time compared to culture\/<jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> based methods.<\/jats:p>","DOI":"10.1111\/myc.12907","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T04:19:07Z","timestamp":1551154747000},"page":"513-518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["A high\u2010throughput and rapid method for accurate identification of emerging multidrug\u2010resistant <i>Candida auris<\/i>"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5029-9536","authenticated-orcid":false,"given":"Ausaf","family":"Ahmad","sequence":"first","affiliation":[{"name":"Enhanced Capacity Surge Laboratory, Reagent and Diagnostic Services Branch Division of Scientific Resources National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Centers for Disease Control &amp; Prevention (CDC)  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan E.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Enhanced Capacity Surge Laboratory, Reagent and Diagnostic Services Branch Division of Scientific Resources National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Centers for Disease Control &amp; Prevention (CDC)  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn R.","family":"Lockhart","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch Division of Foodborne Waterborne and Environmental Diseases National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Centers for Disease Control &amp; Prevention (CDC)  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Singleton","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch Division of Foodborne Waterborne and Environmental Diseases National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Centers for Disease Control &amp; Prevention (CDC)  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Petway","sequence":"additional","affiliation":[{"name":"Enhanced Capacity Surge Laboratory, Reagent and Diagnostic Services Branch Division of Scientific Resources National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Centers for Disease Control &amp; Prevention (CDC)  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Dennis A.","family":"Bagarozzi","sequence":"additional","affiliation":[{"name":"Enhanced Capacity Surge Laboratory, Reagent and Diagnostic Services Branch Division of Scientific Resources National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Centers for Disease Control &amp; Prevention (CDC)  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7007-8207","authenticated-orcid":false,"given":"Owen T.","family":"Herzegh","sequence":"additional","affiliation":[{"name":"Enhanced Capacity Surge Laboratory, Reagent and Diagnostic Services Branch Division of Scientific Resources National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Centers for Disease Control &amp; Prevention (CDC)  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12781"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2404.171715"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2008.00083.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14121"},{"key":"e_1_2_7_6_1","unstructured":"CDC.Tracking Candida auris\u2014August2018.https:\/\/www.cdc.gov\/fungal\/candida-auris\/tracking-c-auris.html. Accessed October 04 2018."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw691"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2018.06.005"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2410.180649"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01355-17"},{"issue":"1","key":"e_1_2_7_11_1","first-page":"195","article-title":"Multidrug\u2010resistant Candida haemulonii and C. auris, Tel Aviv, Israel","volume":"23","author":"Ben\u2010Ami R","year":"2017","journal-title":"Emerg Infect Dis"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00367-15"},{"key":"e_1_2_7_13_1","unstructured":"CDC.Recommendations for Identification of Candida auris.https:\/\/www.cdc.gov\/fungal\/candida-auris\/recommendations.html. 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