{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:42:46Z","timestamp":1790116966559,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Invasive fungal infections caused by filamentous fungi of the order Mucorales are serious complications in immunocompromised patients and often associated with fatal outcome. As a member of this order, <jats:italic>Cunninghamella bertholletiae<\/jats:italic> is a saprophytic fungus with naturally exhibited high minimum inhibitory concentrations against common antifungal drugs and with the potential for outbreaks in clinical settings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives and methods<\/jats:title><jats:p>In a proof\u2010of\u2010principle study, we evaluated the performance of microsatellite markers for the discrimination of thirteen <jats:italic>C.\u00a0bertholletiae<\/jats:italic> isolates from various sources in comparison with a repetitive sequence\u2010based <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (rep\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and random amplification of polymorphic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">RAPD<\/jats:styled-content>). Based on the higher discriminatory power of the microsatellite <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> with five separate primer pairs (Simpson's index of 1 vs 0 [<jats:styled-content style=\"fixed-case\">RAPD<\/jats:styled-content>] and 0 [rep\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>]), the novel method was applied to eight additional isolates, including four well\u2010characterised isolates from a cluster of infections in a next step.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, microsatellite <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> identified 21 separate genotypes. A probable epidemiological association of the cluster isolates could be demonstrated by microsatellite genotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, our findings demonstrate the value of microsatellite <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in genotyping <jats:italic>Cunninghamella bertholletiae<\/jats:italic> and its potential for future applications with other species of the order Mucorales.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12908","type":"journal-article","created":{"date-parts":[[2019,3,2]],"date-time":"2019-03-02T11:44:19Z","timestamp":1551527059000},"page":"519-525","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of genotyping methods for <i>Cunninghamella bertholletiae<\/i>"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5601-2444","authenticated-orcid":false,"given":"Hedda Luise","family":"Verhasselt","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Radke","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Killengray","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Scharmann","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Rickerts","sequence":"additional","affiliation":[{"name":"FG 16: Erreger von Pilz\u2010, und Parasiteninfektionen und Mykobakteriosen, Konsiliarlabor f\u00fcr Kryptokokkose und seltene Systemmykosen Robert Koch\u2010Institut Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiebke","family":"Hansen","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4388-3117","authenticated-orcid":false,"given":"Danila","family":"Seidel","sequence":"additional","affiliation":[{"name":"Department I of Internal Medicine University Hospital of Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5888-7706","authenticated-orcid":false,"given":"Iker","family":"Falces\u2010Romero","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Department Hospital Universitario La Paz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Buer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen 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