{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:40:04Z","timestamp":1790422804609,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,9]],"date-time":"2019-06-09T00:00:00Z","timestamp":1560038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Candida auris<\/jats:italic>, an emerging nosocomial pathogen, exhibits phenotypic variation. Non\u2010aggregating <jats:italic>C. auris<\/jats:italic> isolates display greater biofilm\u2010forming capacity and virulence than aggregate\u2010forming isolates. Most of the studies till date have focused on clinical isolates. The biofilm\u2010forming capacity of colonising isolates remains uninvestigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The present study aimed to elucidate the biofilm\u2010forming capacity of the colonising isolates of <jats:italic>C. auris<\/jats:italic>, correlate it with their aggregation behaviour and antifungal susceptibility, and compare it with that of the isolates from blood\u2010stream infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Colonising and clinical (candidemia) isolates of <jats:italic>C. auris<\/jats:italic> were screened for aggregation behaviour, biofilm\u2010forming capacity and antifungal susceptibility testing. Aggregation behaviour was assessed microscopically. Biofilm\u2010forming capacity was determined on 96\u2010well flat\u2010bottomed microtitre plates. Antifungal susceptibility testing was performed by broth microdilution assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Aggregative and non\u2010aggregative phenotypes were found to be predominantly associated with colonising and clinical isolates, respectively, with the former ones being stronger biofilm producers in the colonising group. Non\u2010aggregative isolates in the colonising group showed lower susceptibility to amphotericin B and fluconazole than aggregative isolates. In contrast, no association was noted between biofilm formation, aggregation behaviour and antifungal susceptibility amongst the clinical isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Biofilm formation is a strain\u2010dependent trait in <jats:italic>C. auris<\/jats:italic>, strongly associated with the type and phenotypic behaviour of the isolates. Colonising isolates of this fungus were found to be predominantly aggregative in nature, with a higher biofilm\u2010forming capacity than non\u2010aggregative ones.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.12947","type":"journal-article","created":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T13:36:01Z","timestamp":1558964161000},"page":"706-709","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Biofilm formation by <i>Candida auris<\/i> isolated from colonising sites and candidemia cases"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9041-0251","authenticated-orcid":false,"given":"Rachna","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Microbial Biotechnology Panjab University Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahaldeep","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Panjab University Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arunaloke","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamanth A.","family":"Shankarnarayan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivaprakash M.","family":"Rudramurthy","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx034"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-17"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2017.08.017"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2017.09.009"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00921-17"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2017.11.015"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.05.007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.493563"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02396-16"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2302.161320"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00334-18"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphereDirect.00680-18"},{"key":"e_1_2_7_14_1","first-page":"e00189\u201016","volume":"1","author":"Borman AM","year":"2016","journal-title":"Comparative Pathogenicity of United Kingdom Isolates of the Emerging Pathogen Candida Auris and Other key Pathogenic Candida Species. MSphere"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000747"},{"key":"e_1_2_7_16_1","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Fourth Informational Supplement. 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