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Although studies using alternative infectious models to evaluate the efficacy of antifungal molecules have been increasingly described and reported, there is no critical consensus that establishes the most appropriate ones regarding the type of infection. Numerous studies contemplate<jats:italic>ex vivo<\/jats:italic>protocols of fungal infections on nails, corneas, dentinal tubules and skin and reveal counterpoints and concordances not yet finely confronted. In this minireview, we propose a critical analysis of the main<jats:italic>ex vivo<\/jats:italic>models of fungal infections for the evaluation of new antifungal candidates for both topical and systemic use, as opposed to the advantages and disadvantages of the traditional in vivo models employed in preclinical research.<\/jats:p>","DOI":"10.1111\/myc.12961","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T18:29:21Z","timestamp":1562264961000},"page":"860-873","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Fungal infection models: Current progress of<i>ex vivo<\/i>methods"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6676-6039","authenticated-orcid":false,"given":"Priscilla","family":"Maciel Quatrin","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Microbiologia Agr\u00edcola e do Ambiente 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