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This study aims the assessment of cytokines and antimicrobial peptides gene expression elicited by <jats:italic>M. sympodialis<\/jats:italic> and <jats:italic>M. furfur<\/jats:italic> once in contact with a reconstructed human epidermis. A yeast suspension was prepared in <jats:styled-content style=\"fixed-case\">RPMI<\/jats:styled-content> 1640 medium (Sigma\u2010Aldrich, St. Louis, <jats:styled-content style=\"fixed-case\">MO<\/jats:styled-content>) supplemented with Tween 60 and oleic acid to obtain approximately 1 \u00d7 10<jats:sup>6<\/jats:sup> cells in a volume of 100\u00a0\u03bcL. Clinical isolates of <jats:italic>M. sympodialis<\/jats:italic> (from pityriasis versicolor) and <jats:italic>M. furfur<\/jats:italic> (from seborrhoeic dermatitis) were inoculated, separately, onto a reconstructed human epidermis. A distinct expression pattern was found between the two tested species, with a tendency for overexpression of pro\u2010inflammatory cytokines very soon after infection, whereas no significant expression or gene downregulation was often noticed following 24 and 48\u00a0h of incubation. A possible <jats:italic>Malassezia<\/jats:italic> species\u2010dependent immune response pattern is highlighted.<\/jats:p>","DOI":"10.1111\/myc.12965","type":"journal-article","created":{"date-parts":[[2019,7,6]],"date-time":"2019-07-06T14:43:26Z","timestamp":1562424206000},"page":"932-936","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["<i>Malassezia<\/i> interaction with a reconstructed human epidermis: Keratinocyte immune response"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5969-0235","authenticated-orcid":false,"given":"Ana F.","family":"Pedrosa","sequence":"first","affiliation":[{"name":"Division of Microbiology Department of Pathology Faculty of Medicine University of Porto Porto Portugal"},{"name":"Department of Dermatology and Venereology Centro Hospitalar S.Jo\u00e3o EPE Porto Portugal"},{"name":"Faculty of Medicine CINTESIS \u2010 Center for Health Technology and Services Research University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Lisboa","sequence":"additional","affiliation":[{"name":"Division of Microbiology Department of Pathology Faculty of Medicine University of Porto Porto Portugal"},{"name":"Department of Dermatology and Venereology Centro Hospitalar S.Jo\u00e3o EPE Porto Portugal"},{"name":"Faculty of Medicine CINTESIS \u2010 Center for Health Technology and Services Research University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Branco","sequence":"additional","affiliation":[{"name":"Division of Microbiology Department of Pathology Faculty of Medicine University of Porto Porto Portugal"},{"name":"Faculty of Medicine CINTESIS \u2010 Center for Health Technology and Services Research University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Pellevoisin","sequence":"additional","affiliation":[{"name":"Episkin Academy Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel Marcos","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of Surgery and Physiology Cardiovascular R&amp;D Centre Faculty of Medicine University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Acacio G.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Division of Microbiology Department of Pathology Faculty of Medicine University of Porto Porto Portugal"},{"name":"Faculty of Medicine CINTESIS \u2010 Center for Health Technology and Services Research University of Porto Porto Portugal"},{"name":"Burn Unit Department of Plastic and Reconstructive Surgery Centro Hospitalar S.Jo\u00e3o EPE Porto 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