{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:12:47Z","timestamp":1766869967513,"version":"3.40.5"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Paracoccidioidomycosis (<jats:styled-content style=\"fixed-case\">PCM<\/jats:styled-content>) is a neglected fungal infection with a high impact on the quality of life of the affected patients. The disease presents primary pulmonary involvement and systemic dissemination may occur. About 50% of the cases show oral involvement, and the factors that lead to this manifestation are not clear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to evaluate the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation profile in <jats:styled-content style=\"fixed-case\">PCM<\/jats:styled-content> patients with oral lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was extracted from whole blood of eighteen <jats:styled-content style=\"fixed-case\">PCM<\/jats:styled-content> patients, being ten with oral lesions and eight with no oral lesion. Analysis of methylation profile was performed using the technique of methylation\u2010sensitive arbitrarily primed <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). The sequences of recombinant plasmids obtained were evaluated according to parameters that define a CpG island, as well as their relative position in the known human genome genes and\/or CpG islands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> amplification, three different expressed bands were observed between the two groups, being found in the samples of patients with no oral manifestations. The cloned fragment in the plasmid showed similarity with a <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequence present in chromosome 20, next to the <jats:styled-content style=\"fixed-case\">YTHDF<\/jats:styled-content>1 gene. Other bands showed homology with intronic region in the genes <jats:styled-content style=\"fixed-case\">RBPMS<\/jats:styled-content>2 and <jats:styled-content style=\"fixed-case\">DPH<\/jats:styled-content>6 and no CpG island was identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation was found in <jats:styled-content style=\"fixed-case\">PCM<\/jats:styled-content> patients with no oral lesion affecting the <jats:styled-content style=\"fixed-case\">YTHDF<\/jats:styled-content>1 gene. Further studies are necessary to elucidate to role of <jats:styled-content style=\"fixed-case\">YTHDF<\/jats:styled-content>1 gene in the oral <jats:styled-content style=\"fixed-case\">PCM<\/jats:styled-content> manifestations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13000","type":"journal-article","created":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T17:44:26Z","timestamp":1568137466000},"page":"1133-1139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Different <scp>DNA<\/scp> methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions"],"prefix":"10.1111","volume":"62","author":[{"given":"Paola","family":"Singi","sequence":"first","affiliation":[{"name":"Department of Clinic and Surgery School of Dentistry Federal University of Alfenas Alfenas MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raissa P.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Graduate Program in Microbiology Federal University of Minas Gerais Belo Horizonte MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina L.","family":"de Carli","sequence":"additional","affiliation":[{"name":"Department of Clinic and Surgery School of Dentistry Federal University of Alfenas Alfenas MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Adolfo C.","family":"Hanemann","sequence":"additional","affiliation":[{"name":"Department of Clinic and Surgery School of Dentistry Federal University of Alfenas Alfenas MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro A. C.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Pathology and Parasitology Institute of Biomedical Sciences Federal University of Alfenas Alfenas MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Felipe L.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Institute of Biomedical Sciences Federal University of Alfenas Alfenas MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4920-072X","authenticated-orcid":false,"given":"Luiz Cosme C.","family":"Malaquias","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Institute of Biomedical Sciences Federal University of Alfenas Alfenas MG 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