{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T05:20:31Z","timestamp":1768022431214,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T00:00:00Z","timestamp":1572912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307510\/2015\u20108"],"award-info":[{"award-number":["307510\/2015\u20108"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Paracoccidioidomycosis (PCM) is highly prevalent in Latin America, but no commercial system is available for diagnosing this endemic mycosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To check the performance of (1\u00a0\u2192\u00a03)\u2010\u03b2\u2010D\u2010glucan assay (BDG) for diagnosing\u00a0 PCM in 29 patients with proven fungal disease and compared with double immunodiffusion assay for detecting anti\u2010<jats:italic>Paracoccidioides<\/jats:italic> antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p>We selected 52 serum samples sequentially obtained from 29 patients with active PCM (12 chronic and 17 acute form). Samples were collected at baseline, and for\u00a016\u00a0patients, additional serum levels were obtained after 3 and 6\u00a0months of antifungal treatment. Detection of BDG in serum was performed by using the Fungitell<jats:sup>\u00ae<\/jats:sup> assay. For the double immunodiffusion assay, <jats:italic>Paracoccidioides<\/jats:italic> exoantigen was used in latex agglutination tests to detect serum anti\u2010<jats:italic>Paracoccidioides<\/jats:italic> antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Despite exhibiting good sensitivity in the diagnosis of patients with PCM, we failed to demonstrate any correlation between the postdiagnosis kinetic profile of BDG serum levels and clinical response to antifungal therapy. This finding may be related to the maintenance of quiescent foci of fungal infection in several organs and tissues, a phenomenon that has been previously reported by other authors and helps to understand why so many relapses are documented in patients treated for short periods of time. Finally, we did not find any correlation between BDG quantification and specific anti\u2010<jats:italic>P brasiliensis<\/jats:italic> antibodies serum titres in patients with PCM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, BDG is detected in serum samples of most patients with PCM but is probably not useful for predicting clinical response to antifungal therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13007","type":"journal-article","created":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T10:11:30Z","timestamp":1568801490000},"page":"38-42","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of (1\u00a0\u2192\u00a03)\u2010\u03b2\u2010D\u2010glucan assay for diagnosing paracoccidioidomycosis"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6900-6468","authenticated-orcid":false,"given":"Analy Salles de Azevedo","family":"Melo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Especial de Micologia Departamento de Medicina Universidade Federal de S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4946-911X","authenticated-orcid":false,"given":"Daniel Wagner de Castro Lima","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Micologia Departamento de Medicina Universidade Federal de S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraia Lopes","family":"Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Micologia Departamento de Medicina Universidade Federal de S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-4563","authenticated-orcid":false,"given":"Anderson Messias","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pat\u00f3genos F\u00fangicos Emergentes Departamento de Microbiologia, Imunologia e Parasitologia Universidade Federal de S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoilo Pires","family":"de Camargo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia M\u00e9dica Departamento de Microbiologia, Imunologia e Parasitologia Universidade Federal de S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm","family":"Finkelman","sequence":"additional","affiliation":[{"name":"Associates of Cape Cod Clinical Development Department Research Laboratory Falmouth MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0793-8491","authenticated-orcid":false,"given":"Arnaldo Lopes","family":"Colombo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Micologia Departamento de Medicina Universidade Federal de S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo 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