{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T15:24:22Z","timestamp":1769268262568,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T00:00:00Z","timestamp":1570320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Poor clinical outcomes for invasive aspergillosis are associated with antifungal resistance. Performing antifungal susceptibility tests on clinically relevant <jats:italic>Aspergillus<\/jats:italic> isolates from patients and environmental regions with known azole resistance is recommended. The aim of the study was to assess the presence of azole resistance in clinical <jats:italic>Aspergillus<\/jats:italic> spp. isolates and those from hospital environments and farmlands within a 40\u00a0km radius of the hospital. Clinical <jats:italic>Aspergillus<\/jats:italic> spp. isolates were cultured, as well as environmental <jats:italic>Aspergillus<\/jats:italic> spp. isolates obtained from air samples. Samples were subcultured in azole\u2010containing agar plates. Isolates with a positive screening test were subjected to YeastOne methods to determine their minimum inhibitory concentrations of antifungals. Resistance mechanisms were investigated in the azole\u2010resistant <jats:italic>Aspergillus<\/jats:italic> spp. isolates. No azole\u2010resistant clinical or environmental <jats:italic>A\u00a0flavus<\/jats:italic>,<jats:italic> A\u00a0oryaze<\/jats:italic>,<jats:italic> A\u00a0niger<\/jats:italic> or <jats:italic>A\u00a0terreus<\/jats:italic> isolates were found in the present study. All <jats:italic>A\u00a0fumigatus<\/jats:italic> clinical isolates were azole\u2010susceptible. Seven <jats:italic>A\u00a0fumigatus<\/jats:italic> environmental isolates were associated with <jats:italic>cyp51A<\/jats:italic> mutations, including two that harboured TR<jats:sub>34<\/jats:sub>\/L98H mutations with S297T\/F495I substitutions, two with TR<jats:sub>34<\/jats:sub>\/L98H mutations and three with TR<jats:sub>46<\/jats:sub>\/Y121F\/T289A mutations. One of these isolates was collected from farmland, one was from A ward and five were from B ward. The proportion of azole\u2010resistant <jats:italic>A\u00a0fumigatus<\/jats:italic> was 10.2% (6\/59) and 3.2% (1\/31) in the hospital environments and the farmlands near the hospital, respectively. The results showed\u00a0that azole\u2010resistant <jats:italic>A\u00a0fumigatus<\/jats:italic> existed within hospital environments. This emphasises the importance of periodic surveillance in hospital environments and monitoring for the emergence of azole\u2010resistant <jats:italic>A\u00a0fumigatus<\/jats:italic> clinical isolates.<\/jats:p>","DOI":"10.1111\/myc.13008","type":"journal-article","created":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T05:10:51Z","timestamp":1569301851000},"page":"1174-1181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Azole resistance in <i>Aspergillus<\/i> species in Southern Taiwan: An epidemiological surveillance study"],"prefix":"10.1111","volume":"62","author":[{"given":"Yi\u2010Chun","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Division of Infectious Diseases Kaohsiung Chang Gung Memorial Hospital Kaohsiung 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