{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:00:38Z","timestamp":1790352038727,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,15]],"date-time":"2019-10-15T00:00:00Z","timestamp":1571097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004160","name":"Mazandaran University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004160","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Aspergillus flavus<\/jats:italic> is a major cause of severe non\u2010invasive fungal infections in the Middle Eastern countries. However, it is difficult to distinguish <jats:italic>A flavus<\/jats:italic> from <jats:italic>A oryzae<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess the potential of matrix\u2010assisted laser desorption\/ionisation time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) in discriminating between <jats:italic>A flavus<\/jats:italic> and <jats:italic>A oryzae<\/jats:italic> and compare it with \u03b2\u2010tubulin gene sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used the Bruker Daltonik MALDI\u2010TOF MS system to analyse 200 clinical and environmental <jats:italic>A flavus<\/jats:italic> isolates and one <jats:italic>A pseudonomius<\/jats:italic> and one <jats:italic>A alliaceus<\/jats:italic> (<jats:italic>Aspergillus<\/jats:italic> section <jats:italic>Flavi<\/jats:italic>) isolate a priori identified as such by sequencing of the \u03b2\u2010tubulin gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 200 <jats:italic>A flavus<\/jats:italic> isolates were identified at the genus level and 176 (88%) at the species levels by MALDI\u2010TOF MS based on the spectral log\u2010scores (\u22652.0 and 1.7\u20101.99, respectively); among them, only 18 (10.2%) were confirmed as <jats:italic>A flavus<\/jats:italic>, whereas 35 (19.9%) were identified as <jats:italic>A oryzae<\/jats:italic> and 123 (69.9%) as <jats:italic>A flavus\/A oryzae<\/jats:italic>. <jats:italic>Aspergillus pseudonomius<\/jats:italic> and <jats:italic>A alliaceus<\/jats:italic> were misidentified as <jats:italic>A flavus<\/jats:italic> and <jats:italic>A parasiticus<\/jats:italic> with log\u2010score values of 1.39 and 1.09, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results indicate that the commercially available Bruker Daltonik MALDI\u2010TOF MS score database cannot separate <jats:italic>A flavus<\/jats:italic> and <jats:italic>A oryzae<\/jats:italic> species. We also showed that establishment of an in\u2010house library is a useful tool to discriminate closely related <jats:italic>Aspergillus<\/jats:italic> species, including <jats:italic>A flavus<\/jats:italic> and <jats:italic>A oryzae<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13010","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T06:06:31Z","timestamp":1569477991000},"page":"1182-1188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Discrimination of <i>Aspergillus flavus<\/i> from <i>Aspergillus oryzae<\/i> by matrix\u2010assisted laser desorption\/ionisation time\u2010of\u2010flight (MALDI\u2010TOF) mass spectrometry"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6415-4648","authenticated-orcid":false,"given":"Mohammad T.","family":"Hedayati","sequence":"first","affiliation":[{"name":"Invasive Fungi Research Center 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