{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:37:33Z","timestamp":1792640253843,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,21]],"date-time":"2019-10-21T00:00:00Z","timestamp":1571616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Biofilm formation represents a major microbial virulence attribute especially at epithelial surfaces such as the skin.\n                      <jats:italic>Malassezia<\/jats:italic>\n                      biofilm formation at the skin surface has not yet been addressed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      The present study aimed to evaluate\n                      <jats:italic>Malassezia<\/jats:italic>\n                      colonisation pattern on a reconstructed human epidermis (RhE) by imaging techniques.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Malassezia<\/jats:italic>\n                      clinical isolates were previously isolated from volunteers with pityriasis versicolor and seborrhoeic dermatitis. Yeast of two strains of\n                      <jats:italic>M\u00a0furfur<\/jats:italic>\n                      and\n                      <jats:italic>M\u00a0sympodialis<\/jats:italic>\n                      were inoculated onto the SkinEthic\n                      <jats:sup>\u2122<\/jats:sup>\n                      RHE. The tissues were processed for light microscopy, wide\u2010field fluorescence microscopy and scanning electron microscopy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Colonisation of the RhE surface with aggregates of\n                      <jats:italic>Malassezia<\/jats:italic>\n                      yeast entrapped in a multilayer sheet with variable amount of extracellular matrix was unveiled by imaging techniques following 24, 48, 72 and 96\u00a0hours of incubation. Whenever yeast were suspended in RPMI medium supplemented with lipids, the biofilm substantially increased with a dense extracellular matrix in which the yeast cells were embedded. Slight differences were found in the biofilm architectural structure between the two tested species with an apparently higher entrapment and viscosity in\n                      <jats:italic>M\u00a0furfur<\/jats:italic>\n                      biofilm.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Skin isolates of\n                      <jats:italic>M\u00a0furfur<\/jats:italic>\n                      and\n                      <jats:italic>M\u00a0sympodialis<\/jats:italic>\n                      were capable of forming biofilm in vitro at the epidermal surface simulating in vivo conditions. Following 24\u00a0hours of incubation, without added lipids, rudimental matrix was barely visible, conversely to the reported at plastic surfaces. The amount of biofilm apparently increased progressively from 48 to 96\u00a0hours. A structural heterogeneity of biofilm between species was found.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.13011","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T02:05:28Z","timestamp":1569463528000},"page":"1194-1201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Malassezia<\/i>\n                    colonisation on a reconstructed human epidermis: Imaging studies"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5969-0235","authenticated-orcid":false,"given":"Ana Filipa","family":"Pedrosa","sequence":"first","affiliation":[{"name":"Division of Microbiology Department of Pathology Faculty of Medicine University of Porto Porto Portugal"},{"name":"Department of Dermatology and Venereology Centro Hospitalar Universit\u00e1rio S.Jo\u00e3o EPE Porto Portugal"},{"name":"CINTESIS\u2010Center for Health Technology and Services Research Faculty of Medicine of the University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8247-8902","authenticated-orcid":false,"given":"Carmen","family":"Lisboa","sequence":"additional","affiliation":[{"name":"Division of Microbiology Department of Pathology Faculty of Medicine University of Porto Porto Portugal"},{"name":"Department of Dermatology and Venereology Centro Hospitalar Universit\u00e1rio S.Jo\u00e3o EPE Porto Portugal"},{"name":"CINTESIS\u2010Center for Health Technology and Services Research Faculty of Medicine of the University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2104-3742","authenticated-orcid":false,"given":"Joana","family":"Branco","sequence":"additional","affiliation":[{"name":"Division of Microbiology Department of Pathology Faculty of Medicine University of Porto Porto Portugal"},{"name":"CINTESIS\u2010Center for Health Technology and Services Research Faculty of Medicine of the University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Chromosome Instability &amp; Dynamics Laboratory Instituto de Biologia Molecular e Celular University of Porto Porto Portugal"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade (i3S) University of Porto Porto Portugal"},{"name":"Graduate Program in Biomedicine Faculty of Medicine University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mendes","sequence":"additional","affiliation":[{"name":"Department of Surgery and Physiology Cardiovascular R&amp;D Centre Faculty of Medicine University of Porto Porto 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