{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:31:17Z","timestamp":1792020677818,"version":"4.4.0"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,2]]},"abstract":"Summary<\/jats:title>\n \n Background<\/jats:title>\n Progressive disseminated histoplasmosis (PDH) is an important cause of mortality in persons living with HIV (PLHIV), especially in countries where patients have limited access to antiretroviral therapies and diagnostic testing.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n A lateral flow assay (LFA) to detect\n Histoplasma capsulatum<\/jats:italic>\n antigen in serum developed by MiraVista\n \u00ae<\/jats:sup>\n was evaluated.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We tested 75 serum samples: 24 from PLHIV and culture\u2010proven PDH and 51 from PLHIV with other fungal and bacterial infections as well as people without HIV. LFA devices were read manually (read by eye) and by an automated reader.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n When the LFA was read manually, sensitivity was 96% and specificity was 90%. When an automated reader was used, sensitivity was 92% and specificity was 94%. The Kappa index comparing manual and automated reader was 0.90. Cross\u2010reactions were observed principally in samples from patients with proven diagnosis of paracoccidioidomycosis.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The MiraVista\n \u00ae<\/jats:sup>\n Diagnostics\n Histoplasma<\/jats:italic>\n antigen LFA had high analytical performance and good agreement between manual and automated reader. This LFA allows\n Histoplasma<\/jats:italic>\n antigen testing with minimal laboratory equipment and infrastructure requirements.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/myc.13023","type":"journal-article","created":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T06:38:33Z","timestamp":1571380713000},"page":"139-144","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Evaluation of a\n Histoplasma<\/i>\n antigen lateral flow assay for the rapid diagnosis of progressive disseminated histoplasmosis in Colombian patients with AIDS"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9809","authenticated-orcid":false,"given":"Diego H.","family":"C\u00e1ceres","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC) Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-1924","authenticated-orcid":false,"given":"Beatriz L.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group School of Medicine and Health Sciences Universidad del Rosario Bogota Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Tob\u00f3n","sequence":"additional","affiliation":[{"name":"Hospital La Maria Medellin Colombia"},{"name":"Universidad CES Medellin Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom M.","family":"Chiller","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC) Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Lindsley","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC) Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,27]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"2949","volume-title":"Principles and Practice of Infectious Diseases","author":"Deepe GS","year":"2015"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mmcr.2017.07.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003290"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0084"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof5030073"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e328306eb8d"},{"key":"e_1_2_8_8_1","first-page":"45","volume-title":"Diagnosis and Treatment of Human Mycoses","author":"Pfeiffer CD","year":"2015"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12877"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01959-17"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00101-14"},{"key":"e_1_2_8_12_1","first-page":"115","volume-title":"Medici\u00f3n en salud: diagn\u00f3stico y evaluaci\u00f3n de resultados: un manual critico m\u00e1s all\u00e1 de lo b\u00e1sico","author":"Orozco LC","year":"2010"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00793-10"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/513647"},{"key":"e_1_2_8_15_1","first-page":"785","article-title":"Epidemiology of endemic systemic fungal infections in Latin America","volume":"49","author":"Colombo AL","year":"2011","journal-title":"Med 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