{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T06:56:45Z","timestamp":1758265005506,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sporotrichosis is a group of zoonotic subcutaneous mycoses, found worldwide and caused by fungi belonging to the genus <jats:italic>Sporothrix.<\/jats:italic> Protozoans of the genus <jats:italic>Acanthamoeba<\/jats:italic> are widely distributed, and some species may be pathogenic and\/or opportunistic. These organisms coexist in the same environment and may interact.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study determined the profile of interactions of <jats:italic>S\u00a0schenckii <\/jats:italic>sensu<jats:italic> stricto<\/jats:italic> and <jats:italic>S\u00a0brasiliensis<\/jats:italic> with <jats:italic>A\u00a0castellanii<\/jats:italic>, using an in vitro co\u2010culture model to evaluate the intrinsic characteristics of the two <jats:italic>Sporothrix<\/jats:italic> species and <jats:italic>A\u00a0castellanii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared the rate of phagocytosis of <jats:italic>S\u00a0schenckii <\/jats:italic>sensu<jats:italic> stricto<\/jats:italic> and <jats:italic>S\u00a0brasiliensis<\/jats:italic> by <jats:italic>A\u00a0castellanii<\/jats:italic>; the viability of <jats:italic>S\u00a0schenckii <\/jats:italic>sensu<jats:italic> stricto<\/jats:italic> and <jats:italic>S\u00a0brasiliensis<\/jats:italic> after contact with <jats:italic>A\u00a0castellanii<\/jats:italic>; the viability of the amoeba after contact with a fungal species; and the influence of <jats:italic>S\u00a0schenckii <\/jats:italic>sensu<jats:italic> stricto<\/jats:italic> and <jats:italic>S\u00a0brasiliensis<\/jats:italic> on the encystment process of <jats:italic>A\u00a0castellanii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The analyses indicated that <jats:italic>A\u00a0castellanii<\/jats:italic> phagocytised both <jats:italic>S\u00a0schenckii<\/jats:italic> and <jats:italic>S\u00a0brasiliensis<\/jats:italic>, with significantly more <jats:italic>S\u00a0schenckii<\/jats:italic> than <jats:italic>S\u00a0brasiliensis<\/jats:italic> in the first two hours of contact<jats:italic>.<\/jats:italic> Our results showed a significant increase in conidia and hyphae count after 72\u00a0hours of co\u2010culture of <jats:italic>A\u00a0castellanii<\/jats:italic> with <jats:italic>S\u00a0brasiliensis<\/jats:italic>, and the amoebae lysed after they ingested the fungi, indicating that the fungi probably used the amoebae as a source of nutrition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results were obtained in vitro and these organisms may not behave similarly in vivo; in vivo studies of co\u2010infections are necessary in order to gain\u00a0a thorough understanding\u00a0of this relationship.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13043","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T06:19:51Z","timestamp":1575958791000},"page":"302-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The interaction between <i>Sporothrix schenckii <\/i>sensu<i> stricto<\/i> and <i>Sporothrix brasiliensis<\/i> with <i>Acanthamoeba 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