{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:23:15Z","timestamp":1790378595582,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T00:00:00Z","timestamp":1586736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001866","name":"Fonds National de la Recherche Luxembourg","doi-asserted-by":"publisher","award":["99302"],"award-info":[{"award-number":["99302"]}],"id":[{"id":"10.13039\/501100001866","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The prevalence of azole resistance in\n                      <jats:italic>C parapsilosis<\/jats:italic>\n                      is very low in most parts of the world. However, South Africa has reported an exceptionally high prevalence of azole resistance in\n                      <jats:italic>C parapsilosis<\/jats:italic>\n                      strains isolated from candidaemia cases. We aimed to determine the possible molecular mechanisms of fluconazole resistance in\n                      <jats:italic>C parapsilosis<\/jats:italic>\n                      isolates obtained through surveillance at a large neonatal unit at a South African academic hospital.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We sequenced the\n                      <jats:italic>ERG11<\/jats:italic>\n                      and\n                      <jats:italic>MRR1<\/jats:italic>\n                      genes of\n                      <jats:italic>C parapsilosis<\/jats:italic>\n                      isolates recovered from cases of neonatal candidemia, followed by microsatellite genotyping. A total of 73 isolates with antifungal susceptibility results were analysed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of these, 57 (78%) were resistant, 11 (15%) susceptible dose\u2010dependent and 5 (7%) susceptible. The most commonly identified amino acid substitution within the\n                      <jats:italic>ERG11<\/jats:italic>\n                      gene was Y132F in 68% (39\/57) of fluconazole\u2010resistant isolates and none in susceptible isolates. Three amino acid substitutions (R405K, G583R and A619V) and 1 nucleotide deletion at position 1331 were identified within\n                      <jats:italic>MRR1<\/jats:italic>\n                      gene in 19 (26%) isolates. Microsatellite genotyping grouped isolates into four clusters (50 isolates). Cluster 1 accounted for 23% (17\/73) of all cases, cluster 2 for 22% (16\/73), cluster 3 for 14% (10\/73) and cluster 4 for 10% (7\/73). We found an association between cluster type and fluconazole resistance (\n                      <jats:italic>P<\/jats:italic>\n                      \u2010value\u00a0=\u00a0.004). Isolates harbouring the Y132F substitution were more likely to belong to a cluster than non\u2010Y132F isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Fluconazole resistance in\n                      <jats:italic>C parapsilosis<\/jats:italic>\n                      strains from a single South African neonatal unit was associated with cluster type and predominantly driven by Y123F amino acid substitutions in the\n                      <jats:italic>ERG11<\/jats:italic>\n                      gene.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.13070","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T02:36:55Z","timestamp":1583203015000},"page":"471-477","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Fluconazole\u2010resistant\n                    <i>Candida parapsilosis<\/i>\n                    strains with a Y132F substitution in the\n                    <i>ERG11<\/i>\n                    gene causing invasive infections in a neonatal unit, South Africa"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0950-0146","authenticated-orcid":false,"given":"Rindidzani E.","family":"Magobo","sequence":"first","affiliation":[{"name":"Centre for Healthcare\u2010Associated Infections Antimicrobial Resistance and Mycoses A Division of the National Health Laboratory Service National Institute for Communicable Diseases Johannesburg South Africa"},{"name":"Faculty of Health Sciences University of the Witwatersrand Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn R.","family":"Lockhart","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7869-9462","authenticated-orcid":false,"given":"Nelesh P.","family":"Govender","sequence":"additional","affiliation":[{"name":"Centre for Healthcare\u2010Associated Infections Antimicrobial Resistance and Mycoses A Division of the National Health Laboratory Service National Institute for Communicable Diseases Johannesburg South Africa"},{"name":"Faculty of Health Sciences University of the Witwatersrand Johannesburg South 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