{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T13:05:15Z","timestamp":1770815115912,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T00:00:00Z","timestamp":1591660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The number of invasive <jats:italic>Candida<\/jats:italic> infections has significantly increased in recent decades. For the successful treatment of fungal infections, rapid identification at the species level, particularly in polyfungal infections, is a key factor.<\/jats:p><jats:p>In this study, four commercially available chromogenic media, Candi<jats:italic>Select<\/jats:italic>\u2122 4 (CS4), chromID\u2122 Candida Agar (CCA), BBL\u2122 CHROMagar\u2122 Candida Medium (BBL) and Brilliance\u2122 Candida Agar (BCA) were evaluated for <jats:italic>Candida<\/jats:italic> identification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material\/Methods<\/jats:title><jats:p>Overall, 181 bronchial secretion samples from intensive care patients were analysed prospectively. In addition, 18 primarily sterile materials, previously tested positive for <jats:italic>Candida<\/jats:italic>, were investigated retrospectively. All samples were cultured as recommended by the manufacturer and visually inspected after 24 and 48\u00a0hours by three independent investigators. As a control, colonies were identified by MALDI\u2010TOF MS. Specificity and sensitivity were determined for <jats:italic>C\u00a0albicans<\/jats:italic> identification prospectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CS4 and BCA showed the best overall consensus with the identification results reached by MALDI\u2010TOF MS for <jats:italic>Candida\u00a0albicans<\/jats:italic> and species. A clear differentiation between the species could be ascertained via easily identifiable, species\u2010specific coloration in contrast to BBL and CCA. Sensitivity for <jats:italic>C\u00a0albicans<\/jats:italic> (n\u00a0=\u00a073) identification varied between 32% (BCA) and 69% (CS4 and CCA) after 24\u00a0hours and 68% (BBL) and 82% (BCA) after 48\u00a0hours incubation, while specificity ranged between 62% (BBL) and 81% (CCA) after 24\u00a0hours and 82% (BBL) and 85% (CS4) after 48\u00a0hours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CS4 and BCA are recommended for routine identification of <jats:italic>Candida<\/jats:italic> species in human samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13119","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T20:35:21Z","timestamp":1590438921000},"page":"823-831","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Comparison of four commercially available chromogenic media to identify <i>Candida albicans<\/i> and other medically relevant <i>Candida<\/i> species"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7689-7799","authenticated-orcid":false,"given":"Ulrike","family":"Scharmann","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Kirchhoff","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie le Saout","family":"Chapot","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Dziobaka","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hedda Luise","family":"Verhasselt","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael","family":"Stauf","sequence":"additional","affiliation":[{"name":"Institute of Clinical Hygiene, Medical Microbiology and Infectiology Paracelsus Medical University Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Buer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joerg","family":"Steinmann","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"},{"name":"Institute of Clinical Hygiene, Medical Microbiology and Infectiology Paracelsus Medical University Nuremberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter\u2010Michael","family":"Rath","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis697"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-014-3408-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101510"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000111"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12038"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.8.1923-1929.1994"},{"key":"e_1_2_9_8_1","unstructured":"bioM\u00e9rieux.chromID\u2122 Candida Agar.https:\/\/www.biomerieux.de\/klinische\u2010diagnostik\/chromidtm\u2010bei\u2010pilzinfektionen. Accessed April 27 2020."},{"key":"e_1_2_9_9_1","unstructured":"Oxoid.Brilliance\u2122 Candida;2008.http:\/\/www.oxoid.com\/pdf\/24085_oxoid_brilliance_candida.pdf. Accessed April 27 2020."},{"key":"e_1_2_9_10_1","unstructured":"Bio\u2010Rad.CandiSelect\u21224;2008.http:\/\/www.bio\u2010rad.com\/webroot\/web\/pdf\/inserts\/CDG\/de\/63746_04_2008_DE.pdf. Accessed April 27 2020."},{"key":"e_1_2_9_11_1","unstructured":"Becton Dickinson GmbH.BD CHROMagar Candida Medium.2014.https:\/\/www.bd.com\/resource.Aspx?IDX=9019. 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