{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:06:27Z","timestamp":1789049187263,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The diagnosis of <jats:italic>Aspergillus<\/jats:italic>\u2010sensitised asthma (ASA) and allergic bronchopulmonary aspergillosis (ABPA) is made using IgE against crude antigens of <jats:italic>A\u00a0fumigatus<\/jats:italic> (cAsp). However, the IgE against cAsp has limitations due to cross\u2010reactivity with other fungi.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the utility of recombinant <jats:italic>A<\/jats:italic>\u00a0<jats:italic>fumigatus<\/jats:italic> (rAsp) antigens in detecting ASA and their role in differentiating true from cross\u2010sensitisation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed IgE against rAsp (f 1, f 2, f 3, f 4 and f 6), cAsp and other fungal (<jats:italic>Alternaria<\/jats:italic>, <jats:italic>Candida<\/jats:italic>, <jats:italic>Cladosporium<\/jats:italic>, <jats:italic>Malassezia<\/jats:italic> and <jats:italic>Trichophyton<\/jats:italic>) antigens in subjects with <jats:italic>A\u00a0fumigatus<\/jats:italic>\u2010unsensitised asthma (Af\u2010UA [n\u00a0=\u00a051]), ASA (n\u00a0=\u00a071) and ABPA (n\u00a0=\u00a0123). The diagnoses were made using cAsp\u2010IgE and compared using rAsp\u2010IgE. Subjects with elevated cAsp\u2010IgE, but negative rAsp f 1 and f 2, were presumed to lack true <jats:italic>A\u00a0fumigatus<\/jats:italic> sensitisation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The prevalence of any rAsp antigen positivity (cut\u2010off, 0.35\u00a0kUA\/L) varied from 2%\u201022%, 32%\u201073% and 84%\u201098% for Af\u2010UA, ASA and ABPA, respectively. The prevalence of sensitisation to other fungi ranged from 29%\u201065%, 59%\u201085% and 87%\u201095%, respectively, among subjects with Af\u2010UA, ASA and ABPA. Nineteen subjects of ASA and one subject with ABPA were positive with cAsp\u2010IgE but negative for rAsp f 1 and f 2 and were also cross\u2010sensitised to at least one of the other fungi. Five subjects of Af\u2010UA (cAsp\u2010IgE negative) were rAsp f 1 or f 2 positive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Crude <jats:italic>Aspergillus<\/jats:italic> antigens may misclassify <jats:italic>Aspergillus<\/jats:italic> sensitisation among asthmatics. IgE against rAsp antigens (f 1 and f 2) potentially detect true <jats:italic>Aspergillus<\/jats:italic> sensitisation and could be used for this purpose.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13124","type":"journal-article","created":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T10:07:20Z","timestamp":1591178840000},"page":"928-936","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Role of recombinant <i>Aspergillus fumigatus<\/i> antigens in diagnosing <i>Aspergillus<\/i> sensitisation among asthmatics"],"prefix":"10.1111","volume":"63","author":[{"given":"Valliappan","family":"Muthu","sequence":"first","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawan","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pulmonary 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Thurai","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashutosh Nath","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4714-6299","authenticated-orcid":false,"given":"Mandeep","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Radiodiagnosis and Imaging Postgraduate Institute of Medical Education and Research (PGIMER) Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arunaloke","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute 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