{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:24:40Z","timestamp":1770971080067,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2016\u20100206"],"award-info":[{"award-number":["PICT 2016\u20100206"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Triazole resistance in <jats:italic>Aspergillus fumigatus sensu stricto<\/jats:italic> due to mutations in the <jats:italic>cyp51A<\/jats:italic> gene has been widely reported. Data from Argentina, and particularly from cystic fibrosis (CF) patients, are limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the prevalence and molecular mechanisms of azole resistance in <jats:italic>A. fumigatus sensu stricto<\/jats:italic> recovered from this population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ninety\u2010three <jats:italic>A. fumigatus<\/jats:italic> isolates from 50 CF patients were retrospectively analysed for azole resistance using the standard microbroth dilution method according to CLSI M38\u2010A2 guidelines. Sequencing analysis of the <jats:italic>cyp51A<\/jats:italic> gene and its promoter region was conducted in those isolates displaying high MIC values to itraconazole, voriconazole and\/or posaconazole.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 14% of isolates displayed high MIC values to at least one azole. Of them, 30.7% had the mutation TR34\u2010L98H. No mutations in the <jats:italic>cyp51A<\/jats:italic> gene or its promoter were found in the remaining non\u2013wild\u2010type strains. Therefore, other mechanisms associated with azole resistance can be highly prevalent in these isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>To the best of our knowledge, this is the first study in Latin America reporting azole\u2010resistant <jats:italic>A. fumigatus<\/jats:italic> strains recovered from respiratory secretions of CF patients. Noteworthy, the prevalence of azole resistance in <jats:italic>A. fumigatus sensu stricto<\/jats:italic> in the studied Argentinean CF population is alarmingly high.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13139","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T11:29:57Z","timestamp":1594380597000},"page":"937-941","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["High prevalence of triazole\u2010resistant <i>Aspergillus fumigatus sensu stricto<\/i> in an Argentinean cohort of patients with cystic fibrosis"],"prefix":"10.1111","volume":"63","author":[{"given":"Tom\u00e1s","family":"Brito Devoto","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM) Universidad de Buenos Aires CONICET Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Hermida\u2010Alva","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM) Universidad de Buenos Aires CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gladys","family":"Posse","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda Hospital Nacional Profesor Alejandro Posadas Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge L.","family":"Finquelievich","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM) Universidad de Buenos Aires CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Garc\u00eda\u2010Effr\u00f3n","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas Argentina"},{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas Universidad Nacional del Litoral Santa Fe Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4467-996X","authenticated-orcid":false,"given":"Mar\u00eda L.","family":"Cuestas","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM) Universidad de Buenos Aires CONICET Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy074"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myz133"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2016.04.022"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-1103"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03429.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-017-0182-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12141"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-017-0190-0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ885"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.14.27"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802545600"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2106.140717"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2018.11.012"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky147"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2014.07.004"},{"key":"e_1_2_8_18_1","unstructured":"Clinical and Laboratory Standards Institute. 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