{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T12:15:31Z","timestamp":1768997731305,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T00:00:00Z","timestamp":1597190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Various tools are obtainable for the detection of <jats:italic>Pneumocystis jirovecii<\/jats:italic>, among them qPCR promising highest sensitivity. A novel molecular method is commercially available, the loop\u2010mediated isothermal amplification (LAMP) assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We compared the performance of the LAMP eazyplex<jats:sup>\u00ae<\/jats:sup> <jats:italic>Pneumocystis jirovecii<\/jats:italic> with the RealStar <jats:italic>Pneumocystis jirovecii<\/jats:italic> PCR 1.0 qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material\/Methods<\/jats:title><jats:p>Overall, 162 lower respiratory tract specimens from 146 critically ill patients were investigated. LAMP assay and qPCR were carried out according to the manufacturer's recommendations. Positive results of the LAMP were described as time to positivity (TTP). The limit of detection (LOD) of the LAMP was analysed using 10\u2010fold serial dilutions of a high positive <jats:italic>P\u00a0jirovecii<\/jats:italic> respiratory sample. For each serial dilution, TTP of the LAMP was plotted against cycle threshold (Ct) values of the qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The LOD of the LAMP was determined to be approximately 4\u00a0\u00d7\u00a010<jats:sup>3<\/jats:sup> copies\/mL. While the LAMP revealed 28 (17%) positive signals from 20 patients, by using qPCR 41 (25%) positive samples from 28 patients were identified. Overall agreement with qPCR was 92%. Five false\u2010negative, one false\u2010positive and nine invalid results were detected by the LAMP. Positive and negative predictive values were 96% each, and sensitivity and specificity were 84% and 99%, respectively. There was a low correlation between the TTP and the fungal load.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The LAMP is a time\u2010saving and easy\u2010to\u2010perform method. It can be used as an alternative diagnostic method. However, for quantification purposes the qPCR is still the gold standard.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13152","type":"journal-article","created":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T14:04:46Z","timestamp":1596290686000},"page":"1107-1114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of a commercial Loop\u2010mediated Isothermal Amplification (LAMP) assay for rapid detection of <i>Pneumocystis jirovecii<\/i>"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7689-7799","authenticated-orcid":false,"given":"Ulrike","family":"Scharmann","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Kirchhoff","sequence":"additional","affiliation":[{"name":"Institute of Medical 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