{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:47:59Z","timestamp":1787316479544,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,9,12]],"date-time":"2020-09-12T00:00:00Z","timestamp":1599868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703136"],"award-info":[{"award-number":["81703136"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81803147"],"award-info":[{"award-number":["81803147"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573060"],"award-info":[{"award-number":["81573060"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      An alternative therapy for sporotrichosis is necessary to reduce the treatment time and raise clinical efficacy. The 5\u2010Aminolevulinic acid\u2010mediated photodynamic therapy (ALA\u2010PDT) is a promising platform with which to treat mycoses. However, despite the worldwide prevalence of\n                      <jats:italic>Sporothrix globosa<\/jats:italic>\n                      , a causative agent of sporotrichosis, the effect of ALA\u2010PDT on this pathogen has not been validated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to evaluate the effect of ALA\u2010PDT on\n                      <jats:italic>S\u00a0globosa<\/jats:italic>\n                      and the protection of melanin through an in vitro study. The mechanisms involved were also investigated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To estimate the survival rate of\n                      <jats:italic>S\u00a0globosa<\/jats:italic>\n                      treated with ALA\u2010PDT and the protection offered by melanin, the conidia and yeast cells of wild\u2010type\n                      <jats:italic>S\u00a0globosa<\/jats:italic>\n                      (Mel+), other clinical strains, tricyclazole\u2010treated Mel+ and an albino mutant strain (Mel\u2212) were incubated with and without ALA or irradiation. Reactive oxygen species generation by Mel+ conidia induced by ALA\u2010PDT was assayed. SEM and TEM were conducted to obverse ultrastructural changes in the conidia. A comet assay was performed to evaluate DNA damage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The survival rate of\n                      <jats:italic>S\u00a0globosa<\/jats:italic>\n                      conidia and yeast cells significantly decreased following incubation with 1.19M ALA and 162\u00a0J\/cm\n                      <jats:sup>2<\/jats:sup>\n                      irradiation in vitro. Melanin was not only capable of protecting the conidia against ALA\u2010PDT, but also against ALA or irradiation alone. After induction by ALA\u2010PDT, alterations in reactive oxygen species generation, DNA damage and ultrastructural changes were observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      ALA\u2010PDT inhibits the survival of\n                      <jats:italic>S\u00a0globosa<\/jats:italic>\n                      conidia in vitro and therefore has potential for the treatment of sporotrichosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.13171","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T02:22:37Z","timestamp":1598581357000},"page":"1311-1320","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["5\u2010Aminolevulinic acid\u2010mediated photodynamic therapy has effective antifungal activity against\n                    <i>Sporothrix globosa<\/i>\n                    in vitro"],"prefix":"10.1111","volume":"63","author":[{"given":"Ruili","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0568-3029","authenticated-orcid":false,"given":"Lei","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of 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