{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:55:41Z","timestamp":1791910541434,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Infectious keratitis is the main cause of preventable blindness worldwide, with about 1.5\u20132.0 million new cases occurring per year. This inflammatory response may be due to infections caused by bacteria, fungi, viruses or parasites. Fungal keratitis is a poorly studied health problem.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to identify a new fungal species by molecular methods and to explore the possible efficacy of the three most common antifungals used in human keratitis in Mexico by performing in vitro analysis. The capacity of this pathogen to cause corneal infection in a murine model was also evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The fungal strain was isolated from a patient with a corneal ulcer. To identify the fungus, taxonomic and phylogenetic analyses (nrDNA ITS and LSU data set) were performed. An antifungal susceptibility assay for amphotericin B, itraconazole and voriconazole was carried out. The fungal isolate was used to develop a keratitis model in BALB\/c mice; entire eyes and ocular tissues were preserved and processed for histopathologic examination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and conclusion<\/jats:title>\n                    <jats:p>\n                      This fungal genus has hitherto not been reported with human keratitis in Mexico. We described a new species\n                      <jats:italic>Purpurecillium roseum<\/jats:italic>\n                      isolated from corneal infection.\n                      <jats:italic>P\u00a0roseum<\/jats:italic>\n                      showed resistance to amphotericin B and itraconazole and was sensitive to voriconazole. In vivo study demonstrated that\n                      <jats:italic>P\u00a0roseum<\/jats:italic>\n                      had capacity to developed corneal infection and to penetrate deeper corneal tissue. The global change in fungal infections has emphasised the need to develop better diagnostic mycology laboratories and to recognise the group of potential fungal pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/myc.13198","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T13:27:45Z","timestamp":1602854865000},"page":"162-173","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<i>Purpureocillium roseum<\/i>\n                    sp. nov. A new ocular pathogen for humans and mice resistant to antifungals"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2718-3420","authenticated-orcid":false,"given":"Rosa Paulina","family":"Calvillo\u2010Medina","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular Universidad Aut\u00f3noma de Quer\u00e9taro Santiago de Quer\u00e9taro Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Gabriela","family":"Ponce\u2010Angulo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Prote\u00f3mica Ocular Instituto de Oftalmolog\u00eda \u201cFundaci\u00f3n de Asistencia Privada Conde de Valenciana\u201d Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7525-0034","authenticated-orcid":false,"given":"Tania","family":"Raymundo","sequence":"additional","affiliation":[{"name":"Laboratorio de Micolog\u00eda 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