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Early and accurate diagnosis of <jats:italic>C.\u00a0auris<\/jats:italic> infections is crucial, and however, the fungus has often been misidentified by commercial systems.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To develop conventional and real\u2010time PCR methods for accurate and rapid identification of <jats:italic>C.\u00a0auris<\/jats:italic> and its discrimination from closely related species by exploiting the uniqueness of certain glycosylphosphatidylinositol (GPI)\u2010modified protein\u2010encoding genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Species\u2010specific primers for two unique putative GPI protein\u2010encoding genes per species were designed for <jats:italic>C.\u00a0auris<\/jats:italic>, <jats:italic>C.\u00a0haemulonii, C.\u00a0pseudohaemulonii, C.\u00a0duobushaemulonii<\/jats:italic>, <jats:italic>C.\u00a0lusitaniae<\/jats:italic> and <jats:italic>C.\u00a0albicans<\/jats:italic>. Primers were blind tested for their specificity and efficiency in conventional and real\u2010time multiplex PCR set\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All primers combinations showed excellent species specificity. In multiplex mode, correct identification was aided by different\u2010sized amplicons for each species. Efficiency of the <jats:italic>C.\u00a0auris<\/jats:italic> primers was validated using a panel of 155 <jats:italic>C.\u00a0auris<\/jats:italic> isolates, including all known genetically diverse clades. In real\u2010time multiplex PCR, different melting points of the amplicons allowed the distinction of <jats:italic>C.\u00a0auris<\/jats:italic> from four related species. <jats:italic>C.\u00a0auris<\/jats:italic> limit of detection was 5\u00a0CFU\/reaction with a threshold value of 32. The method was also able to detect <jats:italic>C.\u00a0auris<\/jats:italic> in spiked blood and serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PCR identification based on unique GPI protein\u2010encoding genes allows for accurate and rapid species identification of <jats:italic>C.\u00a0auris<\/jats:italic> and related species without need for expensive equipment when applied in conventional PCR set\u2010up.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13204","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T16:50:50Z","timestamp":1604163050000},"page":"194-202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Identification of <i>Candida auris<\/i> and related species by multiplex PCR based on unique GPI protein\u2010encoding genes"],"prefix":"10.1111","volume":"64","author":[{"given":"Mar\u00eda","family":"Alvarado","sequence":"first","affiliation":[{"name":"Regional Center for Biomedical Research Castilla\u2010La Mancha Science &amp; Technology Park, University of Castilla\u2013La Mancha Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn","family":"Bartolom\u00e9 \u00c1lvarez","sequence":"additional","affiliation":[{"name":"Complejo Hospitalario Universitario de Albacete, Servicio de Salud de Castilla\u2010La Mancha Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn R.","family":"Lockhart","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eulogio","family":"Valent\u00edn","sequence":"additional","affiliation":[{"name":"GMCA Research Unit, Departamento de Microbiolog\u00eda y Ecolog\u00eda Universidad de Valencia Burjassot Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba Cecilia","family":"Ruiz\u2010Gait\u00e1n","sequence":"additional","affiliation":[{"name":"Severe Infection Research Group Health Research Institute La Fe Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Eraso","sequence":"additional","affiliation":[{"name":"Departamento de Inmunolog\u00eda, Microbiolog\u00eda y Parasitolog\u00eda Universidad del Pa\u00eds Vasco\/Euskal Herriko Unibertsitatea (UPV\/EHU) Bilbao Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3112-6947","authenticated-orcid":false,"given":"Piet W. 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