{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:47:31Z","timestamp":1791557251514,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,13]],"date-time":"2021-02-13T00:00:00Z","timestamp":1613174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Our aim was to evaluate the performance of two galactomannan (GM) assays (Platelia Aspergillus EIA, Bio\u2010Rad<jats:sup>\u00ae<\/jats:sup>, and Aspergillus GM LFA, IMMY<jats:sup>\u00ae<\/jats:sup>) in tracheal aspirate (TA) samples of consecutive critically ill patients with COVID\u201019.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We included critically ill patients, performed GM\u2010EIA and GM\u2010Lateral Flow Assay (GM\u2010LFA) in TA and followed them until development of COVID\u201019\u2010associated pulmonary aspergillosis (CAPA) or alternate diagnosis. CAPA was defined according to the modified AspICU criteria in patients with SARS\u2010CoV\u20102 infection. We estimated sensitivity, specificity, positive and negative predictive values for GM\u2010EIA, GM\u2010LFA, the combination of both or either positive results for GM\u2010EIA and GM\u2010LFA. We explored accuracy using different breakpoints, through ROC analysis and Youden index to identify the optimal cut\u2010offs. We described antifungal treatment and 30\u2010day mortality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 14\/144 (9.7%) patients with CAPA, mean age was 50.35 (SD 11.9), the median time from admission to CAPA was 8\u00a0days; 28.5% received tocilizumab and 30\u2010day mortality was 57%. ROC analysis and Youden index identified 2.0 OD as the best cut\u2010off, resulting in sensitivity and specificity of 57.1% and 81.5% for GM\u2010EIA and 60% and 72.6% for GM\u2010LFA, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The diagnostic performance of GM in tracheal aspirates improved after using a cut\u2010off of 2\u00a0OD. Although bronchoalveolar lavage testing is the ideal test, centres with limited access to bronchoscopy may consider this approach to identify or rule out CAPA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13216","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T08:25:06Z","timestamp":1605947106000},"page":"364-371","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Accuracy of galactomannan testing on tracheal aspirates in COVID\u201019\u2010associated pulmonary aspergillosis"],"prefix":"10.1111","volume":"64","author":[{"given":"Carla M.","family":"Roman\u2010Montes","sequence":"first","affiliation":[{"name":"Clinical Microbiology Laboratory Infectious Diseases Department Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"},{"name":"Department of Infectious Diseases Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areli","family":"Martinez\u2010Gamboa","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Infectious Diseases Department Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulette","family":"Diaz\u2010Lomel\u00ed","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Infectious Diseases Department Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2279-1558","authenticated-orcid":false,"given":"Axel","family":"Cervantes\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Infectious Diseases Department Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Rangel\u2010Cordero","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Infectious Diseases Department Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Sifuentes\u2010Osornio","sequence":"additional","affiliation":[{"name":"Department of Medicine Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Ponce\u2010de\u2010Leon","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Infectious Diseases Department Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"},{"name":"Department of Infectious Diseases Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0292-3442","authenticated-orcid":false,"given":"Maria F.","family":"Gonzalez\u2010Lara","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Infectious Diseases Department Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"},{"name":"Department of Infectious Diseases Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(18)30274-1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30237-X"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202004-1038LE"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13096"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02554-2020"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200307313490519"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800247"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12166"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz1008"},{"key":"e_1_2_9_12_1","first-page":"141","article-title":"Detection of galactomannan in Broncho\u2010Alveolar lavage fluids by Platelia Aspergillus enzyme immunoassay (BioRad Laboratories. 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