{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:50:54Z","timestamp":1789588254698,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["FAPESP 2017\/27265\u20105"],"award-info":[{"award-number":["FAPESP 2017\/27265\u20105"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["CNPq 433276\/2018\u20105"],"award-info":[{"award-number":["CNPq 433276\/2018\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Sporotrichosis is a subcutaneous mycosis caused by <jats:italic>Sporothrix<\/jats:italic> species that affects humans and animals. Little information on the genetic diversity and population structure of the pathogen is available for Brazil, which is needed to design effective strategies to tackle the advance of sporotrichosis in endemic areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We assessed the genetic diversity and mating\u2010type distribution of <jats:italic>Sporothrix<\/jats:italic> isolates recovered from human and feline cases of sporotrichosis in Esp\u00edrito Santo\u2010Brazil to better understand the population structure, epidemiology and diversification of this pathogen, as well as to explore the possible routes of transmission involved in the ongoing outbreaks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In all, 75 <jats:italic>Sporothrix<\/jats:italic> isolates were identified with phenotypic characteristics. Then, fungal DNA extraction was performed, and the species\u2010specific PCR technique was applied, using markers directed to the calmodulin gene. The mating\u2010type idiomorph of species was identified by PCR using primers targeting the <jats:italic>MAT1\u20101<\/jats:italic> and <jats:italic>MAT1\u20102<\/jats:italic> loci.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 75 <jats:italic>Sporothrix<\/jats:italic> isolates, 76% were confirmed as <jats:italic>S brasiliensis<\/jats:italic> and 24% as <jats:italic>S schenckii<\/jats:italic> sensu <jats:italic>stricto. S brasiliensis<\/jats:italic> was more prevalent in the metropolitan area and <jats:italic>S schenckii s. str<\/jats:italic>. in the mountainous region of the state. In both species, the presence of the two sexual idiomorphs was detected, suggesting that they are heterothallic species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data suggest that sporotrichosis takes on an epidemic\u2010urban character involving <jats:italic>S\u00a0brasiliensis<\/jats:italic>. This species in Esp\u00edrito Santo is likely to originate from Rio de Janeiro, as most isolates harbour the same <jats:italic>MAT 1\u20102<\/jats:italic> locus. We confirm that <jats:italic>S\u00a0brasiliensis<\/jats:italic> has significantly broadened its area of occurrence, an essential feature of emerging pathogens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13230","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T21:41:33Z","timestamp":1608327693000},"page":"420-427","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Molecular\u2010based assessment of diversity and population structure of <i>Sporothrix<\/i> spp. clinical isolates from Esp\u00edrito Santo\u2010Brazil"],"prefix":"10.1111","volume":"64","author":[{"given":"Isabela","family":"da Cruz Bahiense Rocha","sequence":"first","affiliation":[{"name":"Infectious Diseases Postgraduate Program Center for Research in Medical Mycology Federal University of Esp\u00edrito Santo (UFES) Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Portella Della","family":"Terra","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Fungal Pathogens Department of Microbiology, Immunology, and Parasitology Discipline of Cellular Biology Federal University of S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Cardoso de Oliveira","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM) Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Lubianca Zanotti","sequence":"additional","affiliation":[{"name":"Cassiano Ant\u00f4nio Moraes University Hospital (HUCAM) UFES Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alo\u00edsio","family":"Falqueto","sequence":"additional","affiliation":[{"name":"Infectious Diseases Postgraduate Program Center for Research in Medical Mycology Federal University of Esp\u00edrito Santo (UFES) Esp\u00edrito Santo Brazil"},{"name":"Cassiano Ant\u00f4nio Moraes University Hospital (HUCAM) UFES Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoilo Pires","family":"de Camargo","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Fungal Pathogens Department of Microbiology, Immunology, and Parasitology Discipline of Cellular Biology Federal University of S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-4563","authenticated-orcid":false,"given":"Anderson Messias","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Emerging Fungal Pathogens Department of Microbiology, Immunology, and Parasitology Discipline of Cellular Biology Federal University of S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo 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