{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T22:11:38Z","timestamp":1781734298575,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T00:00:00Z","timestamp":1622764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20fk0108104"],"award-info":[{"award-number":["JP20fk0108104"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20fk0410016"],"award-info":[{"award-number":["JP20fk0410016"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although fungi are found relatively easily by microscopic examination of pathological samples, identification of fungal genus and species in pathological samples is not easy because the morphological features of fungi are similar among genera and species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>A multiple real\u2010time PCR was developed for identification of fungal genus\/species, and morphological characterizations of fungi were analysed in pathological samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>Seventy\u2010five formalin\u2010fixed paraffin\u2010embedded (FFPE) samples morphologically proven to contain any fungus were examined. A multiple real\u2010time PCR system was developed to identify 25 fungal genus\/species in pathological samples. Morphology of fungus in the specimens was re\u2010reviewed retrospectively based on the results of real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Real\u2010time PCR identified fungal genus\/species in 56 of 75 (74.6%) specimens with histologically proven fungal infection. In 53 specimens of filamentous fungi, <jats:italic>Aspergillus<\/jats:italic> spp. (22 specimens), Cladosporium (8)<jats:italic>, Scedosporium apiospermum<\/jats:italic> (4), <jats:italic>Malassezia sympodialis<\/jats:italic> (1) and <jats:italic>Candida albicans<\/jats:italic> (1) were identified. Pseudohyphae of Candida were confused with filamentous fungus in a case. Morphological observation suggested differences in the presence of septated or non\u2010septated hyphae, the filament size, and the branch angle among genus\/species of filamentous fungi; however, genus\/species was not able to be determined by their morphological features. In 22 specimens of yeasts, real\u2010time PCR allowed for the identification of <jats:italic>Candida albicans<\/jats:italic> (12 specimens), <jats:italic>Candida glabrata<\/jats:italic> (2), Cladosporium (2), <jats:italic>Scedosporium apiospermum<\/jats:italic> (2), <jats:italic>Pichia kudriavzevii<\/jats:italic> (1) and <jats:italic>Aspergillus sydowii<\/jats:italic> (1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data suggest that it is difficult to identify fungal genus\/species by morphological features alone. Real\u2010time PCR is useful to identify fungal genus\/species in pathological samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13325","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T14:29:30Z","timestamp":1621866570000},"page":"851-859","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Morphological and genetic identification of fungal genus\/species in formalin\u2010fixed, paraffin\u2010embedded specimens obtained from patients with histologically proven fungal infection"],"prefix":"10.1111","volume":"64","author":[{"given":"Keishin","family":"Sunagawa","sequence":"first","affiliation":[{"name":"Department of Pathology National Institute of Infectious Diseases Tokyo Japan"},{"name":"Clinical Pathology and Laboratory Medicine, Anatomic Pathology Saitama Cooperative Hospital Saitama 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