{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T20:27:45Z","timestamp":1793305665520,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,7,11]],"date-time":"2021-07-11T00:00:00Z","timestamp":1625961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p><jats:italic>Aspergillus<\/jats:italic> cryptic species are increasingly recognised causes of <jats:italic>Aspergillus<\/jats:italic> diseases, including life\u2010threatening invasive aspergillosis (IA). However, as their accurate identification remains challenging in a routine practice, few is known from a clinical and epidemiological perspective. Recently, the MSI application has emerged as a powerful tool for the detection and identification of <jats:italic>Aspergillus<\/jats:italic> cryptic species. We aimed to use to the network of users of the MSI application to conduct a multicentre prospective screening of <jats:italic>Aspergillus<\/jats:italic> cryptic species\u2010related IA and analyse their epidemiological, clinical and mycological characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Over a 27\u2010month period, the clinical involvement of 369 <jats:italic>Aspergillus<\/jats:italic> cryptic isolates, from 13 French and Danish MSI application users, was prospectively analysed. Species identification was confirmed by DNA\u2010sequencing and antifungal susceptibility testing was performed using EUCAST reference method. Fifty\u2010one <jats:italic>A fumigatus<\/jats:italic> sensu <jats:italic>stricto<\/jats:italic> invasive cases were also analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifteen cryptic isolates were responsible of IA. Eight species were involved, including 5 cases related to the species <jats:italic>A sublatus<\/jats:italic>. These species showed high rate of in vitro low susceptibility to antifungal drugs. In comparison with <jats:italic>A fumigatus<\/jats:italic> sensu <jats:italic>stricto<\/jats:italic> invasive cases, pre\u2010exposure to azole drugs was significantly associated with cryptic IA (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.02).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This study brings new insights in cryptic species related IA and underlines the importance to identify accurately at the species level these <jats:italic>Aspergillus<\/jats:italic> isolates. The increasing use of antifungal drugs might lead in the future to an epidemiologic shift with an emergence of resistant isolates involved in IA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13348","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T19:27:06Z","timestamp":1624908426000},"page":"1346-1353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Invasive aspergillosis due to <i>Aspergillus<\/i> cryptic species: A prospective multicentre study"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4187-0806","authenticated-orcid":false,"given":"Sebastien","family":"Imbert","sequence":"first","affiliation":[{"name":"Service de Parasitologie Mycologie AP\u2010HP Groupe Hospitalier La Piti\u00e9\u2010Salp\u00eatri\u00e8re Paris France"},{"name":"Centre d\u2019Immunologie et des Maladies Infectieuses Sorbonne Universit\u00e9 INSERM CNRS Paris 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