{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:45:54Z","timestamp":1747187154123,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,8,15]],"date-time":"2021-08-15T00:00:00Z","timestamp":1628985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pneumonia due to <jats:italic>Pneumocystis jirovecii<\/jats:italic> (PCP) is a frequent infection in HIV\u2010positive and also in immunocompromised HIV\u2010negative patients. PCR analysis of pulmonary samples has become an essential element in PCP laboratory diagnosis. Currently, many commercially PCR\u2010based tests are available for <jats:italic>P<\/jats:italic>\u00a0<jats:italic>jirovecii<\/jats:italic> detection and need to be evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We evaluated the performance of the RealStar<jats:sup>\u00ae<\/jats:sup> <jats:italic>P\u00a0jirovecii<\/jats:italic> PCR kit for PCP diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed the RealStar<jats:sup>\u00ae<\/jats:sup> <jats:italic>P\u00a0jirovecii<\/jats:italic> PCR and an in\u2010house PCR in 219 pulmonary samples. We then assessed the performance of the RealStar<jats:sup>\u00ae<\/jats:sup> <jats:italic>P\u00a0jirovecii<\/jats:italic> PCR kit by classifying patients in proven, probable, possible PCP or no final diagnosis, on the basis of the clinical and radiological signs and direct examination of bronchoalveolar lavage samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed excellent concordance (96.8%) with another in\u2010house PCR, previously used in the laboratory. The available clinical data allowed classifying 219 patients as having proven PCP (<jats:italic>n<\/jats:italic>\u00a0=\u00a06), probable PCP (<jats:italic>n<\/jats:italic>\u00a0=\u00a027), possible PCP (<jats:italic>n<\/jats:italic>\u00a0=\u00a029) and no final diagnosis of PCP (<jats:italic>n<\/jats:italic>\u00a0=\u00a0157). The RealStar<jats:sup>\u00ae<\/jats:sup> <jats:italic>P\u00a0jirovecii<\/jats:italic> PCR kit performed well with samples from patients with proven and probable PCP, as indicated by the detection of <jats:italic>P<\/jats:italic>\u00a0<jats:italic>jirovecii<\/jats:italic> DNA in all these samples. The percentage of positive samples in the possible PCP category was 75.9%. In patients with no final diagnosis of PCP, <jats:italic>P<\/jats:italic>\u00a0<jats:italic>jirovecii<\/jats:italic> DNA was detected in 13.4% of samples, indicating colonisation by this pathogen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The RealStar<jats:sup>\u00ae<\/jats:sup> <jats:italic>P\u00a0jirovecii<\/jats:italic> PCR kit shows excellent performance for PCP diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13354","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T08:56:51Z","timestamp":1628067411000},"page":"1230-1237","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Performance of the RealStar<sup>\u00ae<\/sup><i>Pneumocystis jirovecii<\/i> PCR kit for the diagnosis of <i>Pneumocystis<\/i> pneumonia"],"prefix":"10.1111","volume":"64","author":[{"given":"Margot","family":"Sals\u00e9","sequence":"first","affiliation":[{"name":"Laboratoire de Microbiologie CHU de N\u00eemes N\u00eemes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Mercier","sequence":"additional","affiliation":[{"name":"MIVEGEC Univ. Montpellier CHU de N\u00eemes, CNRS, IRD Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Jos\u00e9","family":"Carles","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie CHU de N\u00eemes N\u00eemes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Lechiche","sequence":"additional","affiliation":[{"name":"Service de Maladies infectieuses et tropicales CHU de N\u00eemes N\u00eemes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6539-8545","authenticated-orcid":false,"given":"Mil\u00e8ne","family":"Sasso","sequence":"additional","affiliation":[{"name":"MIVEGEC Univ. Montpellier CHU de N\u00eemes, CNRS, IRD Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,15]]},"reference":[{"issue":"5","key":"e_1_2_10_2_1","first-page":"466","article-title":"Projecting the medical costs of AIDS and ARC in the United States","volume":"1","author":"Hay JW","year":"1988","journal-title":"J Acquir Immune Defic 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