{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:28:15Z","timestamp":1790832495584,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Mucorales<\/jats:italic>, as one major order of Zygomycetes fungi, can infect human beings and cause serious consequence. We have noticed the pathogenicity of <jats:italic>Mucorales<\/jats:italic> is closely related to energy metabolism, while mitochondria play the role of energy factories in almost all biological activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Virulence of <jats:italic>M irregularis<\/jats:italic>, <jats:italic>M hiemalis<\/jats:italic>, <jats:italic>L corymbifera<\/jats:italic> and <jats:italic>R arrhizus<\/jats:italic> were verified in <jats:italic>Galleria mellonella<\/jats:italic> larvae, as well as mitochondrial gene copies analysed with RT\u2010qPCR. Mitogenomes of the four <jats:italic>Mucorales<\/jats:italic> species were sequenced based on illumina NovaSeq technology to study their characteristic features and functional regions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Variant virulence of <jats:italic>M irregularis<\/jats:italic>, <jats:italic>M hiemalis<\/jats:italic>, <jats:italic>L corymbifera<\/jats:italic> and <jats:italic>R arrhizu<\/jats:italic> were verified by clinical retrospective data and our <jats:italic>G mellonella<\/jats:italic> infection models, also copies of mitochondrial genes indicated the significant associations with pathogenicity. A total of 274.18 clean reads were generated to be assembled; the complete mitogenomes of the four <jats:italic>Mucorales<\/jats:italic> species were obtained with totally different length. After the genomes annotated and compared, <jats:italic>M irregularis<\/jats:italic> was found more similar with <jats:italic>M hiemalis<\/jats:italic> than those of <jats:italic>L corymbifera<\/jats:italic> and <jats:italic>R arrhizus<\/jats:italic>, especially the small (<jats:italic>rrns<\/jats:italic>) and large (<jats:italic>rrnl<\/jats:italic>) subunits of mitochondrial ribosomal RNA (rRNA) genes. The GC content, ncRNAs and the distribution of the SNPs and InDels were also compared, and the GC content rate of fungi seems to be related to the fungal thermal adaptability. In addition, linear mitogenomes of these four <jats:italic>Mucorales<\/jats:italic> showed diverse arrangements of <jats:italic>orf<\/jats:italic> genes and directionality of some conserved gene elements.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study uncovered the pathogenicity variances among the four <jats:italic>Mucorales<\/jats:italic> species and the relationship between their mitogenomic features and clinical pathogenicity. Further studies like spatial structure of mitochondrial genomes and the comprehensive analysis of transcription regulation are needed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13374","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T21:37:25Z","timestamp":1632778645000},"page":"45-56","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of mitogenomes from four <i>Mucorales<\/i> species and insights into pathogenicity"],"prefix":"10.1111","volume":"65","author":[{"given":"Guanzhao","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Medical Mycology Institute of Dermatology Chinese Academy of Medical Science and Peking Union Medical College Nanjing China"},{"name":"CAMS Collection Center of Pathogen Microorganisms\u2010D (CAMS\u2010CCPM\u2010D\uff09 Nanjing China"},{"name":"Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology Institute of Dermatology Chinese Academy of Medical Science and Peking Union Medical College Nanjing China"},{"name":"CAMS Collection Center of Pathogen Microorganisms\u2010D (CAMS\u2010CCPM\u2010D\uff09 Nanjing China"},{"name":"Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology Institute of Dermatology Chinese Academy of Medical Science and Peking Union Medical College Nanjing China"},{"name":"CAMS Collection Center of Pathogen Microorganisms\u2010D (CAMS\u2010CCPM\u2010D\uff09 Nanjing China"},{"name":"Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai BIOZERON Biotechnology Co., Ltd. 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