{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:37:04Z","timestamp":1792132624587,"version":"4.4.0"},"reference-count":84,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T00:00:00Z","timestamp":1641168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Candida auris<\/jats:italic> is an emerging, potentially multidrug\u2010resistant pathogen that exhibits clade\u2010specific resistance to fluconazole and also develops resistance to echinocandins and amphotericin B easily. This study analysed 49 <jats:italic>C auris<\/jats:italic> isolates for alterations in hotspot\u20101 and hotspot\u20102 of <jats:italic>FKS1<\/jats:italic> for the detection of mutations conferring reduced susceptibility to echinocandins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>C auris<\/jats:italic> isolates (<jats:italic>n<\/jats:italic>\u00a0=\u00a049) obtained from 18 immunocompromised patients during June 2016\u2010December 2018 were analysed. Antifungal susceptibility testing was performed by Etest and broth microdilution\u2010based MICRONAUT\u2010AM assay. Mutations in hotspot\u20101 and hotspot\u20102 regions of <jats:italic>FKS1<\/jats:italic> were detected by PCR sequencing and fingerprinting of the isolates was done by short tandem repeat typing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The patients had multiple comorbidities\/risk factors for <jats:italic>Candida<\/jats:italic> spp. infection including cancer\/leukaemia\/lymphoma\/myeloma (<jats:italic>n<\/jats:italic>\u00a0=\u00a016), arterial\/central line (<jats:italic>n<\/jats:italic>\u00a0=\u00a017), urinary catheter (<jats:italic>n<\/jats:italic>\u00a0=\u00a017), mechanical ventilation (<jats:italic>n<\/jats:italic>\u00a0=\u00a014) and major surgery (<jats:italic>n<\/jats:italic>\u00a0=\u00a09) and received antifungal drugs as prophylaxis and\/or empiric treatment. Seven patients developed <jats:italic>C auris<\/jats:italic> candidemia\/breakthrough candidemia, nine patients had candiduria with\/without candidemia and four patients developed surgical site\/respiratory infection. Resistance to fluconazole and amphotericin B was detected in 44 and four isolates, respectively. Twelve <jats:italic>C auris<\/jats:italic> isolates from eight patients showed reduced susceptibility to echinocandins. Seven isolates contained hostspot\u20101 mutations and three isolates from a candidemia patient contained R1354H mutation in hotspot\u20102 of <jats:italic>FKS1<\/jats:italic>. Ten patients died, five were cured, two were lost to follow\u2010up and treatment details for one patient were not available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings describe development of a novel mutation in <jats:italic>FKS1<\/jats:italic> conferring reduced susceptibility to echinocandins in one patient during treatment and unfavourable clinical outcome for many <jats:italic>C auris<\/jats:italic>\u2010infected patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13419","type":"journal-article","created":{"date-parts":[[2021,12,25]],"date-time":"2021-12-25T11:34:50Z","timestamp":1640432090000},"page":"331-343","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Molecular characterisation of <i>Candida auris<\/i> isolates from immunocompromised patients in a tertiary\u2010care hospital in Kuwait reveals a novel mutation in <i>FKS1<\/i> conferring reduced susceptibility to echinocandins"],"prefix":"10.1111","volume":"65","author":[{"given":"Mohammad","family":"Asadzadeh","sequence":"first","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Kuwait University Safat Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiman","family":"Mokaddas","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Kuwait University Safat Kuwait"},{"name":"Microbiology Department Ibn Sina Hospital Shuwaikh Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3492-4781","authenticated-orcid":false,"given":"Suhail","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Kuwait University Safat Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneesa A.","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Microbiology Department Ibn Sina 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