{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T08:51:04Z","timestamp":1772700664237,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T00:00:00Z","timestamp":1646870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Fungal biomarkers support early diagnosis of invasive fungal infections. In this study, we evaluated the impact of a recent update to the manufacturer\u2010recommended cut\u2010off for beta\u20101,3\u2010D\u2010glucan (BDG) testing (Fujifilm Wako BDG assay) on sensitivity and specificity for the detection of candidemia. Additionally, we compared the performance with tests for <jats:italic>Candida<\/jats:italic> antigen (Ag by Serion ELISA antigen <jats:italic>Candida<\/jats:italic>, Virion\\Serion) and anti\u2010mannan antibodies (Ab by Hemkit Candida IHA, Ravo Diagnostika).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sera of 82 patients with candidemia, which were sampled with a maximum distance of \u00b114\u00a0days from the date of sampling of the corresponding positive blood cultures, were retrospectively analysed for BDG, Ag and Ab. Results of BDG testing were compared with results from sera of 129 patients with candidemia from a different hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sensitivity of BDG testing (47%) was higher than for Ag (17%) or Ab (20%). By combining Ag and Ab testing, sensitivity was raised to 32%. Lowering the cut\u2010off of BDG from 11\u00a0pg\/ml to the newly recommended cut\u2010off of 7\u00a0pg\/ml resulted in a significant increase in sensitivity (47% vs 58%, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.01 and 63% vs 71% <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01). At both centres, the increase was significant in NAC but not in <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>albicans<\/jats:italic> candidemia. No significant effects on specificity were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>BDG testing outperformed Ag and Ab testing and its combination. Lowering the BDG cut\u2010off had no significant impact on specificity. The increase in sensitivity can be mainly attributed to a gain in sensitivity for non\u2010albicans Candida species bloodstream infections.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13421","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T15:24:03Z","timestamp":1642001043000},"page":"500-507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Lower beta\u20101,3\u2010D\u2010glucan testing cut\u2010offs increase sensitivity for non\u2010<i>albicans Candida<\/i> species bloodstream infections"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8138-5891","authenticated-orcid":false,"given":"Johannes","family":"Forster","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Hygiene und Mikrobiologie Julius\u2010Maximilians\u2010Universit\u00e4t W\u00fcrzburg  W\u00fcrzburg 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