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Essential oils (EOs), aromatic natural compounds, are known for their antimicrobial activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this work was to evaluate the antifungal efficacy of seven EOs and a commercial MIX against 10 dermatophytes responsible for OM to select the most effective ones to be included in a preventive or curative formulation based on a green natural nail polish (GNNP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Micro\u2010broth dilution tests in line with EUCAST guidelines and olfactory satisfaction test were performed to select the best natural compounds previously analysed by SPME coupled with GC\u2010MS. The same method was used to evaluate the release over time of the active compounds present in the two modified\u2010GNNPs made by adding the best natural compound selected (the <jats:italic>C. citratus<\/jats:italic> EO) and the MIX. Furthermore, to evaluate the preventive and curative activity of modified\u2010GNNPs, <jats:italic>ex vivo<\/jats:italic> experiments on healthy or colonised nails were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and Conclusions<\/jats:title><jats:p>Data showed that MIX\u2010modified\u2010GNNP had preventive activity as it inhibits the fungal growth by releasing its active ingredients for 7\u2009days, while the OE\u2010modified GNNP acts as a natural drug showing cytocidal activity on nails colonised by dermatophytes, but it requires two weekly applications.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13499","type":"journal-article","created":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T18:03:32Z","timestamp":1658081012000},"page":"1127-1136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Green natural nail polish modified with essential oils to treat onychomycosis"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2991-0855","authenticated-orcid":false,"given":"Maura","family":"Di Vito","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie Universit\u00e0 Cattolica del Sacro Cuore  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Scafuro","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie Universit\u00e0 Cattolica del Sacro Cuore  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melinda","family":"Mariotti","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie Universit\u00e0 Cattolica del Sacro Cuore  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Garzoli","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica e Tecnologie del Farmaco Universit\u00e0 di Roma Sapienza  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Torelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze di Laboratorio e Infettivologiche Fondazione Policlinico Universitario A. 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