{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T11:11:54Z","timestamp":1786360314850,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003970","name":"Isfahan University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003970","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The accurate occurrence rate of <jats:italic>C.\u00a0auris<\/jats:italic> infections is still not clear, mainly due to the defects in detection and identification tools routinely used. In this study, we used conventional PCR and real\u2010time PCR assays for sensitive and specific detection\/identification of <jats:italic>C.\u00a0auris<\/jats:italic> from either yeast isolates or clinical specimens collected from various patients in different parts of Iran. Our survey is the first large\u2010scale study rating the incidence of <jats:italic>C.\u00a0auris<\/jats:italic> infections in Iran.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 439 yeast isolates and 590 clinical specimens were screened by specific <jats:italic>C.\u00a0auris\u2010<\/jats:italic>PCR<jats:italic>,<\/jats:italic> targeting the ITS region. The validity of positive samples was assessed by sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Four out of 590 clinical specimens (0.68%) were positive by conventional PCR, while in real\u2010time PCR performed on 100 clinical samples, including those four samples positive in conventional samples, 6 samples were positive. A complete agreement of the identification of positive cases with sequencing results was documented. Among 439 culture isolates, none was positive for <jats:italic>C.\u00a0auris<\/jats:italic>. After following up and resampling of the patients with positive PCR, only one specimen showed positive culture for <jats:italic>C.\u00a0auris,<\/jats:italic> which was confirmed by sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>C.\u00a0auris<\/jats:italic> is not a common cause of systemic or superficial fungal infections in Iran, and a few detected positive cases can be considered as a commensal, coloniser or infecting yeast which may potentially emerge in some clinical and therapeutical conditions. Mycological and phenotypical assays are not sensitive approaches for isolation\/identification of <jats:italic>C.\u00a0auris<\/jats:italic>, unless a specific and sensitive molecular\u2010based method is applied.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13504","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T07:37:00Z","timestamp":1658389020000},"page":"1137-1145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular investigation of the incidence of <i>Candida auris<\/i> infections at selected hospitals in Iran"],"prefix":"10.1111","volume":"65","author":[{"given":"Fatemeh","family":"Safari","sequence":"first","affiliation":[{"name":"Department of Microbiology, Falavarjan Branch Islamic Azad University  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahboobeh","family":"Madani","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Falavarjan Branch Islamic Azad University  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Kheirollahi","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology, School of Medicine Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Mirhendi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Falavarjan Branch Islamic Azad University  Isfahan Iran"},{"name":"Department of Medical Parasitology and Mycology, School of Medicine; Research Core Facilities Laboratory, Mycology Reference Laboratory Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008921"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2008.00083.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01503-19"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12904"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2019.09.007"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-17"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/615958"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6010030"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00630-17"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.644060"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01223-17"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2018-0227"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12886"},{"issue":"1","key":"e_1_2_10_15_1","first-page":"51","article-title":"First fluconazole\u2010resistant Candida auris isolated from fungal otitis in Iran","volume":"7","author":"Armaki MT","year":"2021","journal-title":"Current Medical Mycology"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-021-00605-6"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13396"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myw004"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-89132012000200020"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01588-17"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02202-16"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7090754"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2019.04.004"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3934\/microbiol.2020017"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001189"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01337-18"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13250"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00630-19"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020157"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.06.003"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1910.130393"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx034"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1595"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6715a6"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw691"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2016.07.008"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13504","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/myc.13504","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T04:23:01Z","timestamp":1692678181000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.13504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,10]]},"references-count":35,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1111\/myc.13504"],"URL":"https:\/\/doi.org\/10.1111\/myc.13504","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,10]]},"assertion":[{"value":"2022-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}