{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T06:54:53Z","timestamp":1778309693188,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:00:00Z","timestamp":1661385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cat\u2010transmitted sporotrichosis (CTS) caused by <jats:italic>Sporothrix brasiliensis<\/jats:italic> has emerged as an important zoonosis in Brazil and neighbouring countries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Evaluate the performance of a lateral flow assay (LFA) for the detection of anti\u2010<jats:italic>Sporothrix<\/jats:italic> antibodies in human sera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A LFA for the detection of anti\u2010<jats:italic>Sporothrix<\/jats:italic> antibodies (Anti\u2010Sporo LFA) in human sera, developed by IMMY, was evaluated using 300 human sera collected prospectively at the Hospital de Cl\u00ednicas, Federal University of Paran\u00e1 (HC\u2010UFPR), in Curitiba, Brazil. These specimens included 100 sera from patients with CTS. CTS cases were classified as follows: 59 lymphocutaneous, 27 fixed cutaneous,13 ocular, and one mixed form. One\u2010hundred specimens from patients with other mycoses, including cryptococcosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a032), candidemia (<jats:italic>n<\/jats:italic>\u00a0=\u00a027), paracoccidioidomycosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a014), aspergillosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a010), histoplasmosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a09), fusariosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a04), lobomycosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a01), chromoblastomycosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a01), mucormycosis (<jats:italic>n<\/jats:italic>\u00a0=\u00a01) and <jats:italic>trichosporonosis<\/jats:italic> (<jats:italic>n\u00a0=\u00a01<\/jats:italic>). And 100 specimens from apparently healthy volunteers (AHV).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Anti\u2010Sporo LFA showed a global sensitivity of 83% (95% confidence interval [CI]\u00a0=\u00a074%\u201390%), a global specificity of 82% (95% CI\u00a0=\u00a076%\u201387%), and accuracy of 82% (95% CI\u00a0=\u00a077%\u201386%). By clinical form sensitivity was as follows: Mixed form 100%, ocular 92%, lymphocutaneous 83% and fixed cutaneous 78%. False\u2010positive results were observed in 11 specimens from people with other mycoses and 26 specimens from AHV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and discussion<\/jats:title><jats:p>This study presents the results of the evaluation of the first lateral flow assay for the detection of anti\u2010<jats:italic>Sporothrix<\/jats:italic> antibodies in human sera. The findings here show evidence that IMMY's Anti\u2010Sporo LFA is a promising tool for the rapid diagnosis of CTS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/myc.13516","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T14:49:31Z","timestamp":1660056571000},"page":"926-934","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A lateral flow assay for the immunodiagnosis of human <scp>cat\u2010transmitted<\/scp> sporotrichosis"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3895-1982","authenticated-orcid":false,"given":"Regielly","family":"Cognialli","sequence":"first","affiliation":[{"name":"Mycology Unit, Hospital de Cl\u00ednicas Federal University of Paran\u00e1  Curitiba Brazil"},{"name":"Postgraduate Program in Internal Medicine and Health Science Federal University of Paran\u00e1  Curitiba 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