{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T16:29:53Z","timestamp":1792772993575,"version":"4.5.0"},"reference-count":7,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,12,25]],"date-time":"2022-12-25T00:00:00Z","timestamp":1671926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,12,25]],"date-time":"2022-12-25T00:00:00Z","timestamp":1671926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mycoses"],"published-print":{"date-parts":[[2023,4]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Since 2020 the World Health Organization (WHO) recommends\n Histoplasma<\/jats:italic>\n antigen detection for the diagnosis of disseminated histoplasmosis (DH) in people living with HIV (PLHIV).\n <\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n \n Here we aimed to optimise the IMMY's Clarus\u00ae\n Histoplasma<\/jats:italic>\n GM enzyme immunoassay (EIA), evaluating the best cut\u2010off in the semi\u2010quantitative (SQ\u2010HGM EIA), also known as \u2018calibrator cut\u2010off procedure\u2019.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n The optimization was done using the quantitative standard procedure (Q\u2010HGM EIA), also known as \u2018standard curve procedure\u2019, as reference test. A retrospective study from an endemic area of DH in southern Brazil was carried out including 264 patients investigated for DH using the test. Receiver Operator Characteristic curve was plotted, and sensitivity and specificity of the SQ\u2010HGM EIA were calculated.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The study included 24 positive (values\u2009\u2265\u20090.20\u2009ng\/ml) and 240 negative patients by the Q\u2010HGM EIA. According to the manufacturer SQ\u2010HGM EIA protocol, the new SQ\u2010HGM EIA cut\u2010off of 0.8 EIA units was validated, resulting in sensitivity and specificity of 88% and 98.7%, respectively.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Our study pioneers and brings important data about the optimization of the\n Histoplasma<\/jats:italic>\n antigen testing for the diagnosis of DH in a population from Southern Brazil. This optimization also reduced the amount of reagents used, lowering the cost associated with testing.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/myc.13554","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T23:02:51Z","timestamp":1670972571000},"page":"304-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Optimization of a commercial\n Histoplasma<\/i>\n galactomannan\n EIA<\/scp>\n test in a population from an endemic area of histoplasmosis in southern Brazil"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4647-4792","authenticated-orcid":false,"given":"Bianca dos Santos","family":"Blan","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Faculdade de Medicina (FAMED) Universidade Federal do Rio Grande (FURG) Rio Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7121-3861","authenticated-orcid":false,"given":"Vanice Rodrigues","family":"Poester","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Faculdade de Medicina (FAMED) Universidade Federal do Rio Grande (FURG) Rio Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana Patricia","family":"Basso","sequence":"additional","affiliation":[{"name":"Hospital Universit\u00e1rio Dr. Miguel Riet Corr\u00eaa Jr Empresa Brasileira de Servi\u00e7os Hospitalares (HU\u2010FURG\/ EBSERH) Rio Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9309-5349","authenticated-orcid":false,"given":"J\u00e9ssica Louise","family":"Benelli","sequence":"additional","affiliation":[{"name":"Hospital Universit\u00e1rio Dr. Miguel Riet Corr\u00eaa Jr Empresa Brasileira de Servi\u00e7os Hospitalares (HU\u2010FURG\/ EBSERH) Rio Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine Ortiz","family":"Sanchotene","sequence":"additional","affiliation":[{"name":"Hospital Universit\u00e1rio Dr. Miguel Riet Corr\u00eaa Jr Empresa Brasileira de Servi\u00e7os Hospitalares (HU\u2010FURG\/ EBSERH) Rio Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9809","authenticated-orcid":false,"given":"Diego H.","family":"Caceres","sequence":"additional","affiliation":[{"name":"IMMY Norman Oklahoma USA"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ Nijmegen The Netherlands"},{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences Universidad del Rosario Bogota Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Doherty","sequence":"additional","affiliation":[{"name":"IMMY Norman Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro Comar\u00fa","family":"Pasqualotto","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Patologia Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA) Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa Orzechowski","family":"Xavier","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Faculdade de Medicina (FAMED) Universidade Federal do Rio Grande (FURG) Rio Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,25]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"416","volume-title":"Protocolo Cl\u00ednico e Diretrizes Terap\u00eauticas para Manejo da Infec\u00e7\u00e3o pelo HIV em Adultos. Minist da Sa\u00fade","author":"Minist\u00e9rio da Sa\u00fade","year":"2018"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2019.07.009"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30373-6"},{"key":"e_1_2_11_5_1","unstructured":"PAHO\/WHO.Guidelines for diagnosing and managing disseminated histoplasmosis among people living with HIV.https:\/\/iris.paho.org\/bitstream\/handle\/10665.2\/52304\/9789275122495_eng.pdf?sequence=1&isAllowed=y(Accessed on 30 Ago 2022)."},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01959-17"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2803.212150"},{"key":"e_1_2_11_8_1","volume-title":"Secretaria de Vigil\u00e2ncia em Sa\u00fade. Boletim epidemiol\u00f3gico HIV\/AIDS","author":"Minist\u00e9rio da Sa\u00fade","year":"2021"}],"container-title":["Mycoses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13554","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/myc.13554","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/myc.13554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T14:05:10Z","timestamp":1792764310000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/myc.13554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,25]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1111\/myc.13554"],"URL":"https:\/\/doi.org\/10.1111\/myc.13554","archive":["Portico"],"relation":{},"ISSN":["0933-7407","1439-0507"],"issn-type":[{"value":"0933-7407","type":"print"},{"value":"1439-0507","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,25]]},"assertion":[{"value":"2022-10-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}